松材线虫虫体特异表达cDNA文库的构建与分析

doi:10.11707/j.1001-7488.20111015

Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (10): 98-103.doi: 10.11707/j.1001-7488.20111015

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cDNA Library Construction and Analysis of Differentially Expressed in Mixed Stage Nematode of Bursaphelenchus xylophilus

Huang Lin, Xu Xuling, Li Chao, Ye Jianren, Wu Xiaoqin

Jiangsu Key Laboratory for Prevention and Management of Invasion Species, Nanjing Forestry University Nanjing 210037

Received:2010-10-15 Revised:2011-05-12 Online:2011-10-25 Published:2011-10-25

Abstract

Abstract:

Identification of nematode feeding and pathogenicity-related genes of Bursaphelenchus xylophilus was critical to reveal the molecular mechanism of the Pine Wood Disease (PWD). In this study, a suppression subtractive hybridization (SSH) cDNA library was constructed from the mixed stage nematode cDNA of B. xylophilus isolate AN19#F1, which was driven by the egg cDNA of AN19#F1. A total of 34 contigs generated from 354 ESTs after cDNA sequencing were obtained. Among them 15 contigs were functionally annotated. Contigs, MixC12, MixC25 and MixC33 had high homology with Peroxidase, Beta-1, 4-endoglucanase, zinc finger family protein in the database, respectively. The genes were considered closely related to the feeding and pathogenicity processes. RT-PCR analysis confirmed the respective of the SSH cDNAs.

Key words: Bursaphelenchus xylophilus, mixed stage nematode, pathogenicity-related gene, suppression subtractive hybridization

CLC Number:

S763.1

Huang Lin;Xu Xuling;Li Chao;Ye Jianren;Wu Xiaoqin. cDNA Library Construction and Analysis of Differentially Expressed in Mixed Stage Nematode of Bursaphelenchus xylophilus[J]. Scientia Silvae Sinicae, 2011, 47(10): 98-103.

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cDNA Library Construction and Analysis of Differentially Expressed in Mixed Stage Nematode of Bursaphelenchus xylophilus

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