云南野生黄牡丹谷胱甘肽转移酶(GST)基因的分离及表达分析

doi:10.11707/j.1001-7488.20141209

Abstract

Abstract:

In this study, 10 unigene sequences that share high homology with glutathione S-transferase(GST) protein involved in plant anthocyanin transfering were obtained from previously-constructed tree peony (Paeonia delavayi var. lutea) petal transcriptome database and named PlGST 1-10. Sequences of Open Reading Frame (ORF) in PlGST 1-10 were amplified with designed specific primers using RT-PCR and RACE techniques, and the amino acid sequences were compared and the phylogenetic tree was analy zed. Results showed that PlGST5 was considered to be an alternative gene which is related to anthocyanin transferring, contained a 675 bp ORF encoding 224 amino acid residues with 2 introns and 3 excons and belonged to phi type GST. The predicted protein sequence of PlGST5 also shared high similarity with other GSTs that are related to anthocyanin transferring, such as VvGST4 (Vitis vinifera), PhAn9 (Petunia hybrida), CkmGST3 (Cyclamen persicum), AtTT19 (Arabidopsis thaliana), ScGST3 (Senecio cruentus ) and DcGSTF2 (Dianthus caryophyllus). The expression patterns of PlGST 5 in different tissues and petals with different flower colors during flower development were investigated using qRT-PCR. The results indicated that PlGST5 had a higher expression level in the tissues containing more anthocyanin. The expression level was the highest when flowers fully opened in P. delavayi var. lutea and P. delavayi, while the expression reached the highest abundance at early developmental stage of P. suffruticosa ‘Zhao Fen' and P. suffruticosa ‘Yu Banbai’. In conclusion, we inferred that PlGST5 might associate with the transfer of anthocyanin in P. delavayi var. lutea and this would provide the basis for insight into the molecular mechanisms underlying tree peony yellow flower pigmentation.

Key words: Paeonia delavayi var. lutea, glutathione S-transferase, anthocyanin, gene clone, gene expression

CLC Number:

S718.46

Shi Qianqian, Zhou Lin, Wang Yan. Isolation and Expression Analysis of GST Gene Encoding Glutathione S-Transferase of Paeonia delavayi var. lutea Wild Population in Yunnan[J]. Scientia Silvae Sinicae, 2014, 50(12): 63-72.

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Isolation and Expression Analysis of GST Gene Encoding Glutathione S-Transferase of Paeonia delavayi var. lutea Wild Population in Yunnan

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