PwEXP1在青杄种子萌发及逆境响应中的表达特征

doi:10.11707/j.1001-7488.20141208

Abstract

Abstract:

The PwEXP1 gene was cloned based on the cDNA library of Picea wilsonii and the EST sequence of PwEXP1 using RACE-PCR method in this study. The full length of PwEXP 1 cDNA was 1 186 bp with an open reading frame (ORF) of 777 bp encoding 259 amino acids. Bioinformatics analysis revealed that the theoretical molecular weight of PwEXP1 was 27.68 kDa with isoelectric point (pI) of 8.07. The protein had a typical structure of the expansin protein: containing 8 conserved Cysteine residues at the N terminal, 4 Tryptophan at the C terminal and a HFD in the middle. With RT-qPCR and RT-PCR the temporal dynamics of expression were conducted. It was found that PwEXP1 gene was expressed in needle, root and stem. In the seed germination process, the expression level of PwEXP1 increased with the germination stage and peaked at 6-10 days. Under different abiotic stress (NaCl, ABA, PEG, low and high temperature), gene expression of PwEXP 1 was investigated by using RT-qPCR in roots and leaves. The expression level of PwEXP 1 was up-regulated in root under NaCl, low and high temperature treatments. Under PEG-induced osmotic stress, the expression of PwEXP1 in roots was down-regulated at the early stage, and then induced after 6 h, while the expression showed an up-down-up trend in leaves. With ABA treatment, the expression of PwEXP1 was slightly repressed in both roots and leaves. The results suggest that PwEXP1 plays important roles in both seed germination and responses to abiotic stresses.

Key words: Picea wilsonii, expansin, stress response, gene expression

CLC Number:

S718.46

Zhang Tong, Li Qiaoling, Zhang Lingyun. Expression Characteristics of PwEXP 1 Gene in Seed Germination and Adversity in Picea wilsonii[J]. Scientia Silvae Sinicae, 2014, 50(12): 56-62.

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Expression Characteristics of PwEXP 1 Gene in Seed Germination and Adversity in Picea wilsonii

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