马尾松根际高效解磷真菌的筛选鉴定及其促生效应

doi:10.11707/j.1001-7488.20140911

Abstract

Abstract:

Phosphorus supply shortage in soil is one of reasons for decline of Pinus massoniana stands in China. Phosphate-solubilizing fungi (PSF) are able to dissolve insoluble phosphorus, which is otherwise unavailable to plants, and transform it into soluble phosphorus. PSF can also promote plant growth to some extent. Since Masson pine stands are facing degradation of soil fertility in southern China, screening high-efficient PSF from rhizospheric soil of the forest and developing phosphate-solubilizing microbial fertilizers are an effective way to improve the soil nutrient and promote growth of the pine trees. To this end, in this work 45 phosphate-solubilizing fungi were isolated from 12 samples of P. massoniana rhizosphere in Nanjing Jiangsu and Huangshan Anhui of Eastern China, including 12 fungi that had strong capability to soluble phosphate. After a test of phosphate-solubilizing capability, two strains with strong phosphate-solubilizing capability were obtained: JP-NJ1 and JP-NJ4. Their solubilization of Ca₃(PO₄)₂ accounted for 1051.69 mg ·L^-1and 872.18 mg ·L^-1, respectively. They belonged to Aspergillus awamori and Penicillium pinophilum, as identified by morphological observation and analysis of 18S rDNA sequences. The two efficient phosphate-solubilizing fungal strains were inoculated on P. massoniana seedlings and it was found that both the fungal suspension and their metabolite could promote growth of P. massoniana obviously and the root weight of P. massoniana was enhanced notably.

Key words: Pinus massoniana, phosphate-solubilizing fungi, phosphate-solubilizing capability, 18S rDNA, effect of plant growth promoting

CLC Number:

S718.81

Wei Wei, Wu Xiaoqin, Qiao Huan. Screening and Identification of Phosphate-Solubilizing Fungi of Pinus massoniana Rhizosphere and Its Application[J]. Scientia Silvae Sinicae, 2014, 50(9): 82-88.

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Screening and Identification of Phosphate-Solubilizing Fungi of Pinus massoniana Rhizosphere and Its Application

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