Abstract: [Obiective] In order to understand composition of endophytic Pestalotiopsis and provide the solid foundation for research on metabolites of endophytic Pestalotiopsis. A survey on diversity of endophytic Pestalotiopsis of 16 plant species of Caesalpiniaceae, Magnoliaceae and Myrtaceae were carried out in Nanning Liangfengjiang Forest Park of Guangxi.[Method] The tissue isolation and molecular techniques were used to preliminarily analyze the correlations between endophytic Pestalotiopsis and plant species, plant parts and seasons from or in which the fungus was isolated.[Result] Results showed that 89 isolates of endophytic Pestalotiopsis were isolated from 16 plant species of Caesalpiniaceae, Magnoliaceae and Myrtaceae, and among the isolates 11 species were identified based on morphological characters. Eight, six, eight and six endophytic Pestalotiopsis species were respectively collected from Melaleuca leucadendra (Myrtaceae), Syzygium polypetaloideum (Myrtaceae), Peltophorum tonkinense (Caesalpiniaceae), and Saraca chinensis (Caesalpiniaceae), in which the most endophytic Pestalotiopsis species colonized, of which the highest isolation frequency was 2.86% from Peltophorum tonkinense. It was also found that colonization frequencies of endophytic Pestalotiopsis were higher on twigs than leaves for most of plant species, and higher in autumn than in other seasons. In comparison, Shannon-Wiener's diversity index of endophytic Pestalotiopsis was the highest in Caesalpiniaceae (2.235 3), the second in Myrtaceae (2.163 5) and the lowest in Magnoliaceae (1.863 7).[Conclusion] It indicated that endophytic Pestalotiopsis had preference colonizing in Peltophorum tonkinense, Saraca chinensis, Erythrophleum fordii, and Melaleuca leucadendra and the colonization frequencies were higher in twigs and its colonization frequencies varied in different seasons, among which the highest frequencies were in autumn.

Key words: Pestalotiopsis, colonization, diversity, Caesalpiniaceae, Magnoliaceae, Myrtaceae