中国落叶松八齿小蠹伴生粘束孢属真菌的鉴定及其系统发育分析

doi:10.11707/j.1001-7488.20160810

Abstract

Abstract: [Objective] In this study, we conducted a systematic investigation and analysis on Graphium spp. (Microascales:Graphiaceae) associated with Ips subelongatus Motschulsky (Coleoptera:Scolytidae) in three allopatric larches in northeast China. The study aimed at providing theoretical basis for controling the composite damages by I. subelongatus from the viewpoint of associated fungus pathogens.[Method] In this study, the associated fungi were separated and purified from the body of I. subelongatus and the gallery tissue from allopatric larches:Larix olgensis and L. principis-rupprechtii. Colonies and morphology characteristics were observed, and DNA sequence comparisons for rRNA-ITS and TEF-1α gene regions were conducted.[Result] Result indicated that two species Graphium laricis K. Jacobs, T. Kirisits & M.J. Wingf. and G. carbonarium Paciura, Z.W. de Beer, X.D.Zhou & M.J. Wingf., were stably associated with I. subelongatus. Moreover, G. laricis is a new record species in China. G. laricis and G. carbonarium were first isolated from I. subelongatus, and formed a new association with I. subelongatus. This finding not only enriched the number of strains, but also expanded the distribution range of G. laricis. Meanwhile, this study increased the number of species of Graphium which were isolated in China. G. carbonarium, which was only found from Pissodes sp. (Coleoptera:Curculionidae) in China, now was also isolated from I. subelongatus in this study. The result in this paper added the host species and extended the distribution of host as well. Moreover, the multi-gene sequences characteristic indicates that G. carbonarium, isolated from different hosts of Pissodes sp. and I. subelongatus, could have subspecies differentiation.[Conclusion] Multiple gene sequence characteristics showed that the associated fungi from host plants and bark beetles were two different ecological niches, suggesting that there would be exist differentiation phenomenon in G. carbonarium. The associations between Ips beetles and Graphium fungi exhibited an ideal insight into completely understanding the symbiotic relationship between I. subelongatus and Graphium fungi, and synergistic effect about harming hosts as well.

Key words: Ips subelongatus, G.laricis, G. carbonarium, new record species, association

CLC Number:

S718.81

Liu Xuewei, Lü Quan, Meng Xianjing, Bai Xuesong, Huang Guojun, Li Xiuling, Zhang Xingyao. Identification and Phylogeny of Graphium spp. (Microascales: Graphiaceae) Associated with Ips subelongatus (Coleoptera: Scolytidae) in China[J]. Scientia Silvae Sinicae, 2016, 52(8): 76-87.

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Identification and Phylogeny of Graphium spp. (Microascales: Graphiaceae) Associated with Ips subelongatus (Coleoptera: Scolytidae) in China

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