干旱和复水对栓皮栎幼苗生长和生理特性的影响

doi:10.11707/j.1001-7488.20140710

Abstract

Abstract:

Effects of drought and rewatering on growth, osmoregulation, and antioxidants of two-year-old potted Quercus variabilis seedlingswere investigated. A weighting method was used to control soil water content, by which produced a different water stress gradient. The results showed that: 1) the plant height, basal diameter, leaf number, dry and fresh weight of root, stem and leaf declined with drought stress. Length of tap and secondary roots increased with the increase of stress degree. 2) Activities of antioxidant enzymes including peroxidase, superoxide dismutase, catalase in leaves elevated firstly and then decreased with the increase in duration and degree of drought stresses. Content of malondialdehyde (MDA) increased, while soluble protein and proline contents increased and then decreased during three-week drought treatment. 3) Under drought stress, the Na⁺ content in root, stem and leaf of Q.variabilis seedlings declined, whereas the content of K⁺ changed little; Zn²⁺ concentration decreased in root and stem, but increased in leaf. 4) After rewatering, activities of the antioxidant enzymes recovered to the control level; but MDA content decreased, with MDA content under severe drought stress was significantly higher than that of control. The content of soluble protein and proline decreased, showing no significant difference with control. Mineral elements in different plant organs also recovered to the control level. Thus, the Q.variabilis seedlings reduced aboveground growth and increased the belowground growth to adapt to drought stress. In addition, under light and moderate drought stresses, the Q.variabilis seedlings protected their leaves from damage through increased activities of antioxidant enzymes and osmotic regulation. With the aggravation of drought stress (duration and stress degree), activities of antioxidant enzymes, contents of soluble protein and proline decreased. After rewatering, physiological parameters of Q.variabilis recovered, indicating that Q.variabilis seedlings have a strong tolerance to adapt to drought stress.

Key words: Quercus variabilis, drought stress, rewatering, protective enzyme, growth, biennial Quercus variabilis seedlings

CLC Number:

S718.43

Cui Yuchuan, Zhang Wenhui, Li Zhiping. Effects of Drought Stress and Rewatering on Growth and Physiological Characteristics of Quercus variabilis Seedlings[J]. Scientia Silvae Sinicae, 2014, 50(7): 66-73.

References

陈立松,刘星辉. 1999. 水分胁迫对龙眼幼苗叶片膜脂过氧化及内源保护体系的影响.武汉植物学研究, 17 (2):105-109.
(Chen L S, Liu X H. 1999. Effects of water strese on leaf membrane lipid peroxidation and endogenous protective systems in longan young seedlings. Journal of Wuhan Botanical Research, 17 (2):105-109. [in Chinese] )
陈贵,胡文玉,谢甫绨,等. 1991. 提取植物体内MDA的溶剂及MDA作为衰老指标的探讨.植物生理学通讯,27 (1):44-46.
(Chen G, Hu W Y, Xie P T, et al. 1991. Solvent for extracting malondialdehyde in plant as an index of senescence. Plant Physiology Communications, 27 (1):44-46. [in Chinese] )
段光明. 1993. 锌对小麦抗旱抗热性的保护机制.山西大学学报:自然科学版,16 (2):213-216.
(Duan G M. 1993. The protective mechanism of zinc on drought and heat resistance of wheat. Journal of Shanxi University, 16 (2):213-216. [in Chinese] )
高婷,张文辉. 2012. 不同瑞典能源柳无性系对干旱胁迫的生理响应.西北植物学报,32 (4):739-744.
(Gao T, Zhang W H. 2012. Physiological responses of different energy willow clones under drought stress. Acta Botanica Boreali-Occidentalia Sinica, 32 (4):739-744. [in Chinese] )
高悦,朱永铸,杨志民,等. 2012. 干旱胁迫和复水对冰草相关抗性生理指标的影响.草地学报,20 (2):336-341.
(Gao Y, Zhu Y Z, Yang Z M, et al. 2012. Effects of drought stress and recovery on antioxidant enzyme activities of agropyron cristatum. Acta Agrestia Sinica, 20 (2):336-341. [in Chinese] )
蒋高明. 2001. 当前植物生理生态学研究的几个热点问题.植物生态学报,25 (5):514-519.
(Jiang G M. 2001. Review on some hot topics towards the researches in the field of plant physioecology. Acta Phytoecologica Sinica, 25 (5):514-519. [in Chinese] )
李田,孙景宽,田家怡,等. 2010. 干旱胁迫对杠柳光合特性及抗氧化酶活性的影响.西北植物学报, 30 (12):2466-2471.
(Li T, Sun J K, Tian J Y, et al. 2010. Photosynthesis characteristics and antioxidant enzyme activity in periploca sepium seedlings under drought stress. Acta Botanica Boreali-Occidentalia Sinica, 30 (12):2466-2471. [in Chinese] )
李鹏鹏,张文辉. 2012. 干旱胁迫与不同储藏时间对杠柳种子萌发的影响.植物研究, 32 (5):567-572.
(Li P P, Zhang W H. 2012. Seed germination of periploca sepium under drought stress and different storage time. Bulletin of Botanical Research, 32 (5):567-572. [in Chinese] )
李合生. 2000.植物生理生化实验原理和技术.北京:高等教育出版社.
(Li H S. 2000. Principles and techniques of plant physiological biochemical experiment. Beijing: Higher Education Press. [in Chinese] )
刘丹. 1990. 水分胁迫下小麦幼苗呼吸及渗透调节物质积累的变化.云南农业大学学报, 51 (1):30-37.
(Liu D. 1990. Changes in respiration and some osmoticas accumulation of wheat seedling under water stress. Journal of Yunnan Agricultural University, 51 (1):30-37. [in Chinese] )
马建华,郑海雷,赵中秋,等. 2001. 植物抗盐机理研究进展.生命科学研究, 5 (3):175-179.
(Ma J H, Zheng H L, Zhao Z Q, et al. 2001. Progress in mechanisms of plant resistance to salt stress. Life Science Research, 5 (3):175-179. [in Chinese] )
孙景宽,张文辉,陆兆华,等. 2009. 干旱胁迫下沙枣和孩儿拳头叶绿素荧光特性研究.植物研究, 29 (2):216-223.
(Sun J K, Zhang W H, Lu Z H, et al. 2009. Chlorophyll fluorescence characteristics of Elaeagnus angustifolia L. and Grewia biloba G.Don var.parviflora(Bge.)Hand.-Mazz. seedlings under drought stress. Seedlings under Drought Stress, 29 (2):216-223. [in Chinese] )
孙继亮,李六林,陶书田,等. 2012. 干旱胁迫和复水对梨幼树生理特性的影响.应用与环境生物学报,18 (2):218-223.
(Sun J L, Li L L, Tao S T, et al. 2012. Effects of drought stress and rewatering on physiological characteristics of pear seedling. Chinese Journal of Applied and Environmental Biology, 18 (2):218-223. [in Chinese] )
单长卷,刘遵春. 2006. 土壤水分对嘎拉苹果幼苗水分生理特性和生长特征的影响.西北林学院学报,21 (2):42-44.
(Shan C J, Liu Z C. 2006. Effect of soil water on water physiology and growth characteristics of gala apple seedlings. Journal of Northwest Forestry University, 21 (2):42-44. [in Chinese] )
单长卷. 2007. 土壤干旱对抗旱品种洛麦9133拔节期生理特性的影响. 麦类作物学报, 27 (5):880-883.
(Shan C J. 2007. Effect of soil drought on physiological characteristics of winter wheat luomai 9133 during jointing stage. Journal of Triticeae Crops, 27 (5):880-883. [in Chinese] )
宋凤斌,刘胜群,童淑媛,等. 2010. 不同耐旱性玉米初生根和次生根中铁、锰、铜、锌、钠含量分析.干旱地区农业研究, 28 (3):169-172,176.
(Song F B, Liu S Q, Tong S Y, et al. 2010. Analysis of the iron,manganese,copper,zinc and sodium of root system between maize genotypes differing in drought tolerance. Agricultural Research in the Arid Areas, 28 (3):169-172,176. [in Chinese] )
汤章城,王育启,吴亚华,等. 1986. 不同抗旱品种高粱苗中脯氨酸累积的差异.植物生理学报,12 (2):154-162.
(Tang Z C, Wang Y Q, Wu Y H, et al. 1986. the difference in proline accumulation between the seedlings of two varieties of sorghum with different drought resistance. Acta Phytophysiologica Sinica, 12 (2):154-162. [in Chinese] )
田野,张焕朝,方升佐. 2003. 盐胁迫下土壤-杨树系统中离子运移与分布特征.南京林业大学学报:自然科学版, 27 (4):5-9.
(Tian Y, Zhang H C, Fang S Z. 2003. Transportation and distribution of K⁺,Na⁺,Ca²⁺ and Mg² in soil-poplar system under NaCl stress. Journal of Nanjing Forestry University, 27 (4):5-9. [in Chinese] )
王良桂,张春霞,彭方仁,等. 2008. 干旱胁迫对几种楸树苗木叶片荧光特性的影响.南京林业大学学报:自然科学版, 32 (6):119-122.
(Wang L G, Zhang C X, Peng F R, et al. 2008. Effects of drought stress on the fluorescence characteristics of four type of Catalpa spp. Journal of Nanjing Forestry University(Natural Sciences Edition), 32 (6):119-122. [in Chinese] )
王晶英,敖红,张杰. 2003. 植物生理生化实验技术与原理.哈尔滨:东北林业大学出版社.
王玮,李德全. 2003. 植物盐分胁迫与水分胁迫的异同.植物生理学通讯, 39 (5):491-492.
汪耀富, 孙德梅, 徐传快,等. 2006. 干旱胁迫对烤烟养分吸收分配及产量品质的影响.干旱地区农业研究, 24 (1):65-69.
(Wang Y F, Sun D M, Xu C K, et al. 2006. Effects of drought stress on absorption and distribution of nutrients and yield and quality of flue-cured tobacco. Agricultural Research in the Arid Areas, 24 (1):65-69. [in Chinese] )
汪洪,金继运,周卫. 2004. 不同土壤水分供应与施锌对玉米水分代谢的影响.植物营养与肥料学报,10 (4):367-373.
(Wang H, Jin J Y, Zhou W. 2004. Effect of zinc application on physiological response of maize to different soil moisture regimes. Plant Nutrition and Fertilizing Science, 10 (4):367-373. [in Chinese] )
谢志玉,张文辉,刘新成. 2010. 干旱胁迫对文冠果幼苗生长和生理生化特征的影响.西北植物学报, 30 (5):948-954.
(Xie Z Y, Zhang W H, Liu X C. 2010. Growth and physiological characteristics of Xanthoceras sorbifolia seedlings under soil drought stress. Acta Botanica Boreali-Occidentalia Sinica, 30 (5):948-954. [in Chinese] )
郁万文,曹帮华,曹福亮. 2007. 干旱、旱盐胁迫对刺槐生长及离子吸收分配的影响.南京林业大学学报:自然科学版, 31 (3):68-72.
(Yu W W, Cao B H, Cao F L. 2007. Effects of drought and drought-NaCl stresses on the growth and ionic absorption and distribution of robinia pseudoacacia clones. Journal of Nanjing Forestry University(Natural Sciences Edition), 31 (3):68-72. [in Chinese] )
张志良,瞿伟菁. 2003. 植物生理学实验指导.北京:高等教育出版社.
赵丽英,邓西平,山仑. 2005. 活性氧消除系统对干旱胁迫响应机制.西北植物学报,25 (2):413-418.
(Zhao L Y, Deng X P, Shan L. 2005. The response mechanism of active oxygen species removing system to drought stress. Acta Botanica Boreali-Occidentalia Sinica, 25 (2):413-418. [in Chinese] )
赵玉蓉,王维中. 1995. 植物在盐害下的渗透调节.徐州师范学院学报, 13 (4):59-62.
(Zhao Y R, Wang W Z. 1995. The researches on osmotic-adjustments of plants under salt stress. Journal of Xuzhou Normal University(Natural Science Edition), 13 (4):59-62. [in Chinese] )
Arndt S K, Clifford S C, Wanek W, et al. 2001. Physiological and morphological adaptations of the fruit tree Ziziphus rotundifolia in response to progressive drought stress. Tree Physiology, 21:705-715.
Arora A, Sairam R K, Srivastava G C. 2002. Oxidative stress and antioxidative system in plants. Current science, 82:1227-1238.
Bhargava B S, Raghupati H B. 1998. Analysis of plant material for macro and micronutrients//Tandon HLS(ed). Methods of analysis of soils, plants, waters and fertilizers. Fertilizer Development and Consultation Organisation, New Delhi, India.
de Carvalho M H C. 2008. Drought stress and reactive oxygen species: production, scavenging and signaling. Plant signaling & Behavior, 3(3):156-165.
Timsina J E, Humphreys. 2006. Performance of CERES-rice and CERES-wheat models in rice-wheat systems: a review. Agricultural systems, 90:5-31.
Renault S, Croser C, Franklin J A, et al. 2001. Effects of NaCl and Na₂SO₄ on red-osier dogwood (Cornus stolonifera Michx) seedlings. Plant and Soil, 233 (2):261-268.
Trevor E, Kraus R, Austin F. 1994. Paclobutrazol protects wheat seedlings from heat and paraquat injury is detoxification of active oxygen involved. Plant Cell Physiology, 35 (1):45-52.

[1]	Zhao Zhijiang, Guo Wenxia, Kang Dongwei, Cui Li, Zhao Lianjun, Li Junqing. Response of Radial Growth of Abies faxoniana and Picea purpurea to Climatic Factors in Subalpine of Western Sichuan [J]. Scientia Silvae Sinicae, 2019, 55(7): 1-16.
[2]	Jia Raozhen, Wang Ming, Wang Chuanhua. Growth, Survival and Physiological Response of Food Lichens Usnea longissima of Rhinopithecus roxellana to Simulated Nitrogen Deposition [J]. Scientia Silvae Sinicae, 2019, 55(7): 17-26.
[3]	Wang Yi, Jia Zhongkui, Ma Lüyi, Deng Shixin, Zhu Zhonglong, Sang Ziyang. Effects of Four Plant Growth Regulators on Rooting of the Softwood Cutting of Magnolia wufengensis [J]. Scientia Silvae Sinicae, 2019, 55(7): 35-45.
[4]	Shen Le, Xu Jianmin, Li Guangyou, Lu Zhaohua, Yang Xueyan, Zhu Ying, Hu Yang, Song Peining, Guo Wenzhong. Genetic Parameters for Growth Traits in Eucalyptus urophylla×E. grandis F₁ Hybrids [J]. Scientia Silvae Sinicae, 2019, 55(7): 68-76.
[5]	Xue Chunquan, Xu Qihu, Lin Liping, He Xiao, Cao Lei, Li Haikui. Biomass Dynamic Predicting for Schima superba in Guangdong Based on Allometric and Theoretical Growth Equation [J]. Scientia Silvae Sinicae, 2019, 55(7): 86-94.
[6]	Wu Lifang, Wei Xiaomei, Lu Weidong. Embryonic Callus Induction and Somatic Embryogenesis of Sophora davidii [J]. Scientia Silvae Sinicae, 2019, 55(7): 170-177.
[7]	Jiang Zheng, Yu Qiannan, Qiao Mingfeng, Xiao Juan, Zhang Ziliang, Yin Huajun. Effects of Root Exudates from Picea asperata Seedlings on the Seed Germination and Seedling Growth of Two Herb Species [J]. Scientia Silvae Sinicae, 2019, 55(6): 160-166.
[8]	Wang Yilin, Wang Weifeng, Zhang Yunxiang, Chang Shujun, Guo Jinping. Responses of Leaf Morphological Structure and Physiological Characteristics of Populus euramericana cv. ‘BYu’ to Drought Stress [J]. Scientia Silvae Sinicae, 2019, 55(4): 42-50.
[9]	Guan Jiyuan, Fan Weiguo. Relationship Between Growth and Root Morphological Characteristics of Rosa roxbunghii Seedlings and Endogenous Hormone Content Under Different Phosphorus Levels [J]. Scientia Silvae Sinicae, 2019, 55(4): 51-61.
[10]	Xia Yongjie, Pang Yong, Liu Luxia, Chen Bowei, Dong Bin, Huang Qingfeng. Forest Height Growth Monitoring of Cunninghamia lanceolata Plantation Using Multi-Temporal Aerial Photography with the Support of High Accuracy DEM [J]. Scientia Silvae Sinicae, 2019, 55(4): 108-121.
[11]	He Huaijiang, Zhang Zhonghui, Zhang Chunyu, Hao Minhui, Yao Jie, Xie Zhe, Gao Haitao, Zhao Xiuhai. Short-Term Effects of Thinning Intensity on Stand Growth and Species Diversity of Mixed Coniferous and Broad-Leaved Forest in Northeastern China [J]. Scientia Silvae Sinicae, 2019, 55(2): 1-12.
[12]	Jiang Cheng, Zhou Houjun, Zhao Yanqiu, He Hui, Chu Liwei, Song Xueqin, Lu Mengzhu. Effects of CDD Gene on the Growth and Development of Populus alba×P. glandulosa ‘84K’ in Response to Drought and Salt Stresses [J]. Scientia Silvae Sinicae, 2019, 55(2): 33-40.
[13]	Jiang Xiaoxu, Li Guolei, Shi Wenhui, Zhao Kaifen, Li Cheng. Effects of Cotyledon Loss Intensity and Time on Seedling Growth Status and Reserves Translocation in Quercus variabilis [J]. Scientia Silvae Sinicae, 2018, 54(8): 56-64.
[14]	Qin Aili, Jian Zunji, Ma Fanqiang, Guo Quanshui, Zheng Xiangkun. Effects of the Mother Tree Age, Growth Regulator, Containers and Substrates on Softwood Cutting Propagation of Thuja sutchuenensis [J]. Scientia Silvae Sinicae, 2018, 54(7): 40-50.
[15]	Zhang Jiangtao, Yang Shuhong, Zhu Di, Zhu Yanlin, Liu Youquan. Physiological Response of Annual Grafted Seedlings of Poplar 2025 and Its Two Bud Mutation Varieties to Drought Stress and Evaluation of Drought Resistance [J]. Scientia Silvae Sinicae, 2018, 54(6): 33-43.

Effects of Drought Stress and Rewatering on Growth and Physiological Characteristics of Quercus variabilis Seedlings

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments