云杉幼树根系分泌物对2种草本植物种子萌发和幼苗生长的影响

doi:10.11707/j.1001-7488.20190619

Abstract

Abstract: [Objective] This study aimed to investigate the ecological effects of root exudates input on herb species from seed germination and seedling growth.[Method] We performed an experiment on acceptors(Trifolium repens and Medicago sativa) in which in situ exudates were collected from Picea asperata seedlings.[Result] 1) Root exudates decreased the germination rate, germination vigor and germination index of the two herb species seeds, and significant differences were observed on the M. sativa seedlings. 2) Similarly, both the fresh weight and dry weight of two herb species seedlings were decreased under root exudates treatments, and significant differences were also observed on M. sativa seedlings. 3) Root exudates additions reduced the total length, total surface area and total volume of two herb species seedlings. Among them, significant differences were found only for the total length. 4) Synthesis effect was a vital index of allelopathy. Further analysis showed that the synthesis effects of root exudates on seed germination and seedling growth of two herb species seedlings were negative. And the allelopathy to M. sativa was stronger than that to T. repens. 5) The analysis of root exudates showed that numerous phenolic acids were found in the root exudates of P. asperata seedlings.[Conclusion] 1) Our result manifested that the root exudates from P. asperata seedlings had obvious inhibitory effects on the seed germination and seedling growth of the herb species. 2) Numerous phenolic acids in the root exudates maybe one of major reasons for the observed inhibiting effects of root exudates.

Key words: root exudates, seed germination, seedling growth, subalpine coniferous plantation

CLC Number:

S718.43

Jiang Zheng, Yu Qiannan, Qiao Mingfeng, Xiao Juan, Zhang Ziliang, Yin Huajun. Effects of Root Exudates from Picea asperata Seedlings on the Seed Germination and Seedling Growth of Two Herb Species[J]. Scientia Silvae Sinicae, 2019, 55(6): 160-166.

References

陈龙池, 汪思龙. 2003. 杉木根系分泌物化感作用研究. 生态学报, 23(2):393-398.
(Chen L C, Wang S L. 2003. Preliminary study of allelopathy of root exudates of Chinese fir. Acta Ecologica Sinica, 23(2):393-398.[in Chinese])
崔翠, 蔡靖, 张硕新. 2013. 核桃根系分泌物化感物质的分离与鉴定. 林业科学, 49(2):54-60.
(Cui C, Cai J, Zhang S X. 2013. Isolation and identification of the allelochemicals in walnut (Juglans regia) root exudates. Scientia Silvae Sinicae, 49(2):54-60.[in Chinese])
段剑, 杨洁, 康金林, 等. 2015. 马尾松根际土壤提取物的化感作用. 生态学杂志, 34(3):773-780.
(Duan J, Yang J, Kang J L, et al. 2015. Allelopathic effect of extracts from Pinus massoniana rhizosphere soil. Chinese Journal of Ecology, 34(3):773-780.[in Chinese])
段文标, 王丽霞, 陈立新, 等. 2013. 红松阔叶混交林林隙大小及光照对草本植物的影响. 应用生态学报, 24(3):614-620.
(Duan W B, Wang L X, Chen L X, et al. 2013. Effects of forest gap size and light intensity on herbaceous plants in Pinus koraiensis dominated broadleaved mixed forest. Chinese Journal of Applied Ecology, 24(3):614-620.[in Chinese])
何为, 杨雪英, 肖娟,等. 2017. 氮素富集对青藏高原东缘窄叶鲜卑花灌丛根系分泌物碳输入的影响. 植物生态学报, 41(6):610-621.
(He W, Yang X Y, Xiao J, et al. 2017. Effects of nitrogen enrichment on root exudative carbon inputs in Sibiraea angustata shrubbery at the eastern fringe of Qinghai-Xizang Plateau. Chinese Journal of Plant Ecology, 41(6):610-621.[in Chinese])
孔垂华, 徐效华, 梁文举, 等. 2004. 水稻化感品种根分泌物中非酚酸类化感物质的鉴定与抑草活性. 生态学报, 24(7):1317-1322.
(Kong C H, Xu X H, Liang W J, et al. 2004. Non-phenolic allelochemicals in root exudates of an allelopathic rice variety and their identification and weed-suppressive activity. Acta Ecologica Sinica, 24(7):1317-1322.[in Chinese])
李光, 白文斌, 任爱霞. 2017. 高粱不同连作年限对其根系分泌物组成和化感物质含量的影响. 生态学杂志, 36(12):3535-3544.
(Li G, Bai W B, Ren A X. 2017. Effects of continuous cropping duration of sorghum on components of root exudates and contents of allelochemicals. Chinese Journal of Ecology, 36(12):3535-3544.[in Chinese])
林开敏, 洪伟, 俞新妥, 等. 2001. 杉木人工林林下植物生物量的动态特征和预测模型. 林业科学, 37(zk):99-105.
(Lin K M, Hong W, Yu X T, et al. 2001. The dynamic characteristics and forecasting models of biomass of undergrowth plant in Chinese fir plantation. Scientia Silvae Sinicae, 37(zk):99-105.[in Chinese])
刘芳黎, 张越, 吴富勤, 等. 2017. 自毒和森林凋落物化感作用对极小种群野生植物大树杜鹃种子萌发的影响. 西北植物学报, 37(6):1189-1195.
(Liu F L, Zhang Y, Wu F Q, et al. 2017. Effect of autotoxicity and litter allelopathy on seed germination of Rhododendron protistum var. giganteum, a plant species with extremely small populations in China. Acta Botanica Boreali-Occidentalia Sinica, 37(6):1189-1195.[in Chinese])
刘庆, 吴彦, 何海. 2001. 中国西南亚高山针叶林的生态学问题. 世界科技研究与发展, 23(2):63-69.
(Liu Q, Wu Y, He H. 2001. Ecological problems of subalpine coniferous forest in the southwest of China. World Sci-Tech Research and Development, 23(2):63-69.[in Chinese])
陆茜, 张金池, 孟苗婧. 2017. 长期连栽杨树林根际土壤自毒作用的生物测定. 生态学报, 37(12):4053-4060.
(Lu Q, Zhang J C, Meng M J. 2017. Bioassay for inhibitory autotoxicity of rhizosphere soil under long-term successive monoculture poplar plantations. Acta Ecologica Sinica, 37(12):4053-4060.[in Chinese])
马履一, 李春义, 王希群, 等. 2007. 不同强度间伐对北京山区油松生长及其林下植物多样性的影响. 林业科学, 43(5):1-9.
(Ma L Y, Li C Y, Wang X Q, et al. 2007. Effects of thinning on the growth and the diversity of undergrowth of Pinus tabulaeformis plantation in Beijing mountainous areas. Scientia Silvae Sinicae. 43(5):1-9.[in Chinese])
孟兆鑫. 2007. 川西亚高山森林群落物种多样性与群落结构研究. 四川农业大学学报, 25(4):441-446.
(Meng Z X. 2007. A study on community structure and species diversity of subalpine forests in western Sichuan. Journal of Sichuan Agricultural University, 25(4):441-446.[in Chinese])
沈慧敏, 郭鸿儒, 黄高宝. 2005. 不同植物对小麦、黄瓜和萝卜幼苗化感作用潜力的初步评价. 应用生态学报, 16(4):740-743.
(Shen H M, Guo H R, Huang G B. 2005. Allelopathy of different plants on wheat, cucumber and radish seedlings. Chinese Journal of Applied Ecology, 16(4):740-743.[in Chinese])
孙婧珏, 孙跃志. 2013. 林木根系分泌物的化感作用. 世界林业研究, 26(3):21-26.
(Sun J Y, Sun Y Z. 2013. Allelopathy of tree's root exudates. World Forestry Research, 26(3):21-26.[in Chinese])
涂书新, 吴佳. 2010. 植物根系分泌物研究方法评述. 生态环境学报, 19(9):2493-2500.
(Tu S X, Wu J. 2010. A review on research methods of root exudates. Ecology and Environmental Sciences, 19(9):2493-2500.[in Chinese])
王健敏, 刘娟, 陈晓鸣, 等. 2010. 云南松天然林及人工林群落结构和物种多样性比较. 林业科学研究, 23(4):515-522.
(Wang J M, Liu J, Chen X M, et al. 2010. Comparison of community structures and species diversity in natural forests and forest plantation of Pinus yunnanensis. Forest Research, 23(4):515-522.[in Chinese])
王延平, 王华田. 2010. 植物根分泌的化感物质及其在土壤中的环境行为. 土壤通报, 41(2):501-507.
(Wang Y P, Wang H T. 2010. Allelochemicals from roots exudation and its environment behavior in soil. Chinese Journal of Soil Science, 41(2):501-507.[in Chinese])
卫云燕, 尹华军, 刘庆, 等. 2011. 夜间增温和施肥对川西亚高山针叶林两种树苗根际效应的影响. 生态学报, 31(3):698-708.
(Wei Y Y, Yin H J, Liu Q, et al. 2011. Responses on rhizosphere effect of two subalpine coniferous species to night-time warming and nitrogen fertilization in western Sichuan, China. Acta Ecologica Sinica, 31(3):698-708.[in Chinese])
吴林坤, 林向民, 林文雄. 2014. 根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望. 植物生态学报, 38(3):298-310.
(Wu L K, Lin X M, Lin W X. 2014. Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates. Chinese Journal of Plant Ecology, 38(3):298-310.[in Chinese])
谢星光, 陈晏, 卜元卿, 等. 2014. 酚酸类物质的化感作用研究进展. 生态学报, 34(22):6417-6428.
(Xie X G, Chen Y, Bu Y Q, et al. 2014. A review of allelopathic researches on phenolic acids. Acta Ecologica Sinica, 34(22):6417-6428.[in Chinese])
张茂新,凌冰,孔垂华,等. 2002. 薇甘菊挥发油的化感潜力. 应用生态学报, 13 (10):1300-1302.
(Zhang M X, Ling B, Kong C H, et al. 2002. Allelopathic potential of volatile oil from Mikania micrantha. Chinese Journal of Applied Ecology, 13(10):1300-1302.[in Chinese])
张晓珂, 姜勇, 梁文举, 等. 2004. 小麦化感作用研究进展. 应用生态学报, 15(10):1967-1972.
(Zhang X K, Jiang Y, Liang W J, et al. 2004. Research advances in wheat (Triticum aestivum) allelopathy. Chinese Journal of Applied Ecology, 15(10):1967-1972.[in Chinese])
郑丽, 冯玉龙. 2005. 紫茎泽兰叶片化感作用对10种草本植物种子萌发和幼苗生长的影响. 生态学报, 25(10):2782-2787.
(Zheng L, Feng Y L. 2005. Allelopathic effects of Eupatorium adenophorum Spreng. on seed germination and seedling growth in ten herbaceous species. Acta Ecologica Sinica, 25(10):2782-2787.[in Chinese])
Bertin C, Yang X H, Weston L A. 2003. The role of root exudates and allelochemicals in the rhizosphere. Plant and Soil, 256(1):67-83.
Brzostek E R, Greco A, Drake J E, et al. 2013. Root carbon inputs to the rhizosphere stimulate extracellular enzyme activity and increase nitrogen availability in temperate forest soils. Biogeochemistry, 115(1/3):65-76.
Cheng W X, Parton W J, Gonzalez-Meler M A, et al. 2014. Synthesis and modeling perspectives of rhizosphere priming. New Phytologist, 201(1):31-44.
Chon S U, Choi S K, Jung S, et al. 2002. Effects of alfalfa leaf extracts and phenolic allelochemicals on early seedling growth and root morphology of alfalfa and barnyard grass. Crop Protection, 21(10):1077-1082.
Duke S O. 2007. Weeding with allelochemicals and allelopathy-a commentary. Pest Management Science, 63(4):307-307.
Huang Z Q, Liao L P, Wang S L, et al. 2000. Allelopathy of phenolics from decomposing stump-roots in replant Chinese fir woodland. Journal of Chemical Ecology, 26(9):2211-2219.
Kong C H, Li H B, Hu F, et al. 2006. Allelochemicals released by rice roots and residues in soil. Plant and Soil, 288(1-2):47-56.
Turk M A, Tawaha A M. 2003. Allelopathic effect of black mustard (Brassica nigra L.) on germination and growth of wild oat (Avena fatua L.). Crop Protection, 22(4):673-677.
Williamson G B, Richardson D. 1988. Bioassays for allelopathy:measuring treatment responses with independent controls. Journal of Chemical Ecology, 14(1):181-187.
Wu H, Haig T, Pratley J, et al. 2001. Allelochemicals in wheat (Triticum aestivum L.):cultivar difference in the exudation of phenolic acids. Journal of Agricultural and Food Chemistry, 49(8):3742-3745.
Yin H J, Li Y F, Xiao J, et al. 2013. Enhanced root exudation stimulates soil nitrogen transformations in a subalpine coniferous forest under experimental warming. Global Change Biology, 19(7):2158-2167.

[1]	Luo Xiaoman, Ding Guijie, Wang Yi. Active Constituents of Soil Extracts from Mycorrhizal Seedling Rhizosphere of Pinus massoniana and Their Effects on Seed Germination [J]. Scientia Silvae Sinicae, 2018, 54(8): 32-38.
[2]	Jiang Xiaoxu, Li Guolei, Shi Wenhui, Zhao Kaifen, Li Cheng. Effects of Cotyledon Loss Intensity and Time on Seedling Growth Status and Reserves Translocation in Quercus variabilis [J]. Scientia Silvae Sinicae, 2018, 54(8): 56-64.
[3]	Zhang Junjie, Chai Shengfeng, Wei Xiao, Lv Shihong, Wu Shaohua. Germination Characteristics of the Seed of a Rare and Endangered Plant, Garcinia paucinervis [J]. Scientia Silvae Sinicae, 2018, 54(4): 174-185.
[4]	Shi Wenhui, Li Guolei, Su Shuchai, Liu Yong, Jia Liming, Shang Zhiguo. Combined Effects of Cotyledon Excision and Nursery Fertilization on Field Performance of Quercus variabilis Container Seedlings [J]. Scientia Silvae Sinicae, 2018, 54(1): 64-73.
[5]	Zhang Peng, Zhao Tongtong, Li Mingyue, Wu Lingdong, Shen Hailong. Effects of CaCl₂, ABA or Sucrose Pretreatment on Germination of Dormancy Released Seeds of Fraxinus mandshurica Dehydrated at Low Temperature [J]. Scientia Silvae Sinicae, 2017, 53(7): 54-61.
[6]	Chen Xiangbo, Liu Yang, Zhao Mingshui, Tu Shuping. Embryo Development and Dormancy Releasing of Acer yangjuechi, the Extremely Endangered Plant [J]. Scientia Silvae Sinicae, 2017, 53(4): 65-73.
[7]	Zhang Peng, Song Boyang, Wu Lingdong, Shen Hailong. Germination and Physiological Responses of Dormancy Released Seeds of Fraxinus mandshurica to Dehydration in Different Conditions [J]. Scientia Silvae Sinicae, 2017, 53(3): 60-67.
[8]	Cheng Zhongqian, Li Guolei. Effects of Nitrogen Supply and Container Size on Seedling Growth, Root Characteristics, and Nutrient Status in Quercus variabilis Container Seedlings [J]. Scientia Silvae Sinicae, 2016, 52(4): 21-29.
[9]	Yan Fang, Zhang Enhe, Wang Qinli, Mao Zhuhong. The Mechanisms of Seed Dormancy of Wild Daphne giraldii and Methods for Dormancy Breaking [J]. Scientia Silvae Sinicae, 2016, 52(4): 30-37.
[10]	Zhang Jianhong, Chen Zhiying, Ruan Xiao, Zhang Yuzhu, Pan Cunde, Wang Qiang. Change of Endogenous Plant Hormones Contents during Seedling Growth of Picea schrenkiana Treated with DHAP [J]. Scientia Silvae Sinicae, 2014, 50(4): 121-128.
[11]	Sun Miao, Yang Zhouting, Zhang Cunli, Wei Anzhi, Shi Wei. Hydro-Priming Technique and Its Resistance Physiology Effect for Sea Buckthorn Seed [J]. Scientia Silvae Sinicae, 2014, 50(12): 32-39.
[12]	Han Wenjuan, Cao Xuping, Zhang Wenhui. Effect of Ground Cover on Early Regeneration of Pinus tabulaeformis Plantation [J]. Scientia Silvae Sinicae, 2014, 50(1): 49-54.
[13]	Wang Xuefeng;Hirafuji Masayuki. Modeling the Relationship between Yellow Horn Seedling Growth and Soil Moisture Content [J]. Scientia Silvae Sinicae, 2013, 49(4): 70-76.
[14]	Cui Cui;Cai Jing;Zhang Shuoxin. Isolation and Identification of the Allelochemicals in Walnut (Juglans regia) Root Exudates [J]. , 2013, 49(2): 54-60.
[15]	Liu Jinxian;Guo Qingxue;Wang Yuping;He Ze;Zhang Tengda;Liu Wendan;Tao Jianping. Effects of Bamboo Leaf Litter on the Germination of Four Canopy Trees [J]. , 2013, 49(1): 33-40.

Effects of Root Exudates from Picea asperata Seedlings on the Seed Germination and Seedling Growth of Two Herb Species

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments