毛白杨和烟草次生维管发育相关NAC转录因子的比较

doi:10.11707/j.1001-7488.20140304

Abstract

Abstract: Tobacco (Nicotiana tabacum) plants have typical secondary vascular development process. It is useful for identifying key genes in the secondary vascular development based on the transcriptome comparison between tobacco and Populus tomentosa. Totally 43 pairs of NAC orthologous genes were identified using BLASTP search in the entire Populus trichocarpa genome sequence with NAC transcription factor genes identified in tobacco vascular tissue transcriptome database as probe and were divided into 19 subgroup based on the sequence homology. The analyses of gene expression levels obtained using RNA-seq in tobacco and P. tomentosa suggest that there are 13 pairs of NAC orthologous genes sharing similar expression patterns in tobacco and P. tomentosa. Eight pairs of NAC orthologous genes were selected to perform expression analysis using relative real time RT-PCR in different tissues of tobacco and P. tomentosa. The results indicated that the 8 pairs of NAC orthologous genes shared similar expression patterns in tobacco and P. tomentosa. It is suggested feasibility to study the functions of corresponding poplar genes through studying functions of tobacco NAC transcription factor using tobacco VIGS system and the study provides the basic data for studying the vascular tissue development regulation.

Key words: Nicotiana tabacum, Populus tomentosa, NAC, vascular development

CLC Number:

S718.46

Guo Wei, Lu Mengzhu. Comparison of NAC Transcription Factor Involved in Secondary Vascular Development between Populus tomentosa and Tobacco[J]. Scientia Silvae Sinicae, 2014, 50(3): 22-30.

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Comparison of NAC Transcription Factor Involved in Secondary Vascular Development between Populus tomentosa and Tobacco

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