油茶果糖-1, 6-二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析

doi:10.11707/j.1001-7488.20131123

Abstract

Abstract:

Fructose-1,6-diphosphate aldolase (FBA) in plants is not only one of the key regulatory enzyme in glycolysis pathway but also provide substrate source for two key components of oil synthesis. In this paper, a new FBA full-length cDNA of Camellia oleifera seed was isolated and cloned by RACE technology, and was named CoFBA4 (GenBank number: JX914590). CoFBA4 had an open reading frame with 1 185 base pairs encoding 394 amino acids. Sequence analysis showed that CoFBA4 had nearest genetic distance with FBA of Arabidopsis thaliana, and belonged to subfamily A. CoFBA4 contained glycosylation and fructose-1,6-diphosphate aldolase activity site, but had no transmembrane domain. CoFBA4 belonged to hydrophilic protein. Subcellular localization analysis showed that CoFBA4 acted out of cell. There was a positive relationship between expression abundance of CoFBA4 gene and oil yield from different superior clones during the same maturity period.

Key words: Camellia oleifera, fructose-1,6-diphosphate aldolase, gene cloning, gene expression, subcellular localization

CLC Number:

S718.46

Zeng Yanling, Tan Xiaofeng, Jiang Yao, Liu Min, Wang Jianyong, Zhou Junqin. Molecular Characterization and Expression Analysis of Fructose-1, 6-Diphosphate Aldolase Gene (CoFBA4) from Camellia oleifera[J]. Scientia Silvae Sinicae, 2013, 49(11): 164-170.

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Molecular Characterization and Expression Analysis of Fructose-1, 6-Diphosphate Aldolase Gene (CoFBA4) from Camellia oleifera

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