川硬皮肿腿蜂毒液蛋白的分离纯化

doi:10.11707/j.1001-7488.20131017

Abstract

Abstract:

To explore the parasitic regulatory mechanism of parasitism by Sclerodermus sichuanensis on the host, Tenebrio molitor, the venom injection and Sephadex G75 chromatography were conducted to investigate the effects of venom on the host pupa in the parasitic process. Additionally, a parasitic venom protein was isolated. The results showed that the venom had paralytic effects, and an approximate 51.17 ku paralytic venom protein was isolated and characterized. The paralytic degree had a positive correlation with the concentration of venom injection, and the number of recovered pupa had a negative correlation with the concentration. T. molitor pupa was in slight and reversible paralysis and its exuviate was inhibited when injected with 0.01 VRE (venom reservoir equivalent) venom artificially. The parplysis was non-reversible and complete when the concentration of injected venom rose to 0.2 VRE. Six kinds of macromolecular venom proteins were found in the venom, and among them an approximate 51.17 ku venom protein vpr 4 had paralytic activity, but the paralytic activity was unbalance.

Key words: Sclerodermus sichuanensis, paralysis, venom protein, purification

CLC Number:

S718.7

Zhuo Zhihang, Yang Wei, Qin Huan, Yang Chunping, Yang Hua, Xu Danping. Isolation and Purification of the Venom Proteins in Sclerodermus sichuanensis[J]. Scientia Silvae Sinicae, 2013, 49(10): 106-112.

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Isolation and Purification of the Venom Proteins in Sclerodermus sichuanensis

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