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Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (10): 59-64.doi: 10.11707/j.1001-7488.20061011

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The Extraction and Content Measurement of Acidic Polysaccharides from Eucommia ulmoides Leaves

Dong Juan'e1,Liang Zongsuo1,Jin Aixian2,Yu Zhenyu1,Cheng Mihong1   

  1. 1. Northwest Sci-Tech University of Agricuture and Forestry Yangling 712100;2. Academy of Forest Inventory and Planning,State Forestry Administration Beijing 100714
  • Received:2006-03-29 Revised:1900-01-01 Online:2006-10-25 Published:2006-10-25

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Abstract:

The extraction techniques and a simple measurement method for the acidic polysaccharides from Eucommia ulmoides leaves were studied. The results were as follows:The residue of E.ulmoides leaves after extracting bioactive constituents could be used to extract acidic polysaccharides,which was the multilevel exploitation and reuse of E.ulmoides. The optimum extraction condition was 1.0% ammonia solvent,at the temperature of 100 ℃,2 times and 2 h per time. The acidic polysaccharides could be purified with resin. To measure the contents of acidic polysaccharides with DNS method,the hydrolyzed time should be within 15～20 min,the optimum coloration time was 10 min,the maximum absorption wavelength was 492 nm,and the recovery was 98.30%,RSD,1.76%.

Key words: Eucommia ulmoides leave, acidic polysaccharides, extraction and purification, content measurement