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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (10): 113-117.doi: 10.11707/j.1001-7488.20131018

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Distribution Pattern of Microbes in the Deciduous Broad-Leaved Forest Soil and Screening of Functional Bacteria in Wolong Nature Reserve

Han Mei, Jiao Ruzhen, Dong Yuhong   

  1. Research Institute of Forestry, CAF Beijing 100091
  • Received:2012-05-22 Revised:2013-07-18 Online:2013-10-25 Published:2013-11-05

Abstract:

By using plate cultivation technique, the soil microbial community in different soil layers was studied under deciduous broad-leaved forest in Wolong Nature Reserve, Sichuan. The results showed that the number of bacteria, fungi and actinomycetes in the different soil layers was in the order as FH layer>A layer>B layer>C layer. The numbers of different microbes in all the sample plots were as the follows: bacteria>actinomycetes>fungi. With the selective media of sodium carboxymethyl cellulosea, 28 strains of cellulose-decomposing microorganisms were screened. By applying aniline plate decolorization method and guaiacol color ring method, we found 2 strains of bacteria, 25 strains of fungi and 3 actinomycetes that possess lignin peroxidase and manganese peroxidase, and 5 fungi with laccase. In the culture medium with Ca3(PO4)2 as the sole source of phosphur, 97 strains of phosphur-dissolving bacteria were screened. In the culture medium without nitrogen, 2 strains of nitrogen fixing bacteria were found. Both the number of strains and the amount of phosphur-dissolving bacteria were higher than that of lignocellulose decomposing microorganisms and lignin-decomposing microorganisms.

Key words: deciduous broad-leaved forest, microflora, screening of functional bacteria

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