Abstract:

Suspension-cultured cell lines of Xanthoceras Sorbifolia were established by using callus induced from mature embryos. Growth characteristics of the cell cultures were further studied. The results showed that the optimum initial amount of inoculum amount was 2 g in 40 mL liquid culture medium, and the optimum concentration of plant hormones was 0.5 mg·L^-1 2,4-D and 0.5 mg·L^-1 6-BA and 0.5 mg·L^-1 NAA, respectively. The highest cell mitotic index appeared on day 10,the cell activity was highest on day 3,and the fresh weight peaked on day 14.

Key words: Xanthoceras sorbifolia, suspension culture, inoculum amount, hormones, growing characteristic of cell