蒿柳成花过程中内源激素和多胺含量变化特征

doi:10.11707/j.1001-7488.20180805

Abstract

Abstract: [Objective] In order to analyze the mechanism of flower organ formation and sex determination in dioecious plants, and provide theoretical support for the artificial regulation of flower organ formation and sex differentiation, the endogenous hormones and polyamines contents during the transition process from vegetative to reproductive growth were studied, and their regulation on flower bud differentiation and gender difference in male and female Salix. viminalis were investigated.[Method]The growth process of annual shoots of S. viminalis was divided into three stages:vegetative growth stage, flower bud physiological differentiation stage and flower bud morphological differentiation stage. In this paper, the 15-20-year-old female and male S. viminalis trees were used, and the contents of ABA, ZT, IAA, GA₃, Put, Spm and Spd in the annual shoot tips were determined using HPLC-MS/MS Q-TRAP at different growth stages, and relationship between changes of the endogenous hormones and polyamines with gender differences were analyzed. [Result] In the process from the vegetative growth stage to the flower bud physiological differentiation stage, the contents of ABA, ZT, Put, Spm and Spd and ABA/GA₃ and ZT/GA₃ ratios in male and female S. viminalis, and the IAA/ZT ratio in the females significantly increased; the IAA/ABA ratio in male and female S. viminalis and the GA₃ content in males significantly decreased. The contents of Put, Spm and Spd in male and female S. viminalis, ZT/GA₃ ratio in males and IAA/ABA ratio in females significantly increased from the flower bud physiological differentiation stage to the flower bud morphological differentiation stage. There were significant gender differences between male and female in IAA, Spd contents and IAA/ABA ratio during S1 stage, in ABA, ZT, IAA, Spd contents and IAA/ABA ratio during S2 stage, and in ABA content and ABA/GA₃, ZT/GA₃, IAA/ABA, IAA/ZT ratios during S3 stage. [Conclusion] High levels of ABA, ZT, IAA, PAs, ABA/ GA₃ and ZT/GA₃ could initiate flower bud physiological differentiation and higher levels of PAs, ZT/GA₃ and IAA/ABA could benefit the flower bud morphological differentiation in male and female S. viminalis. During the process of transition from vegetative growth to reproductive growth, although the trends of endogenous hormones and polyamines are similar in female and male S. viminalis, their contents of BA, ZT, IAA, Spd and IAA/ABA ratio appear significantly gender different at certain stages of development, which may be related to sex determination.

Key words: Salix viminalis, flowering, endogenous hormones, ploymines, gender difference

CLC Number:

S718.43

Peng Xiangyong, Cheng Yunhe, Li Zhenjian, Yu Yongchang, Zou Junzhu, Sun Zhenyuan. Variations of Endogenous Hormones and Polymines during Flowering Process in Male and Female Salix viminalis[J]. Scientia Silvae Sinicae, 2018, 54(8): 39-47.

References

崔凤芝, 张运涛. 1996. 多胺与园艺植物生长发育的关系. 河北农业大学学报, 19(3):94-98.
(Cui F Z, Zhang Y T. 1996.Polyamines in relation to growth and development of horticultural crops.Journal of Agricultural University of Hebei, 19(3):94-98. [in Chinese])
何见, 蒋丽娟, 李昌珠, 等. 2009. 光皮树花芽分化过程中内源激素含量变化的研究. 中国野生植物资源, 28(2):41-45.
(He J, Jiang L J, Li C Z, et al. 2009.Changes of endogenous hormones, during the flower bud differentiation of Cornus wilsonian.Chinese Wild Plant Resources, 28(2):41-45. [in Chinese])
刘宗莉, 林顺权, 陈厚彬. 2007. 枇杷花芽和营养芽形成过程中内源激素的变化. 园艺学报, 34 (2):339-344.
(Liu Z L, Lin S Q, Chen H B. 2007. Time course changes of endogenous hormone levels during the floral and vegetativebuds formation in loquat (Eriobotrya japonica Lindl.). Acta Horticulturae Sinica, 34 (2):339-344. [in Chinese])
罗平源, 史继孔, 张万萍. 2006. 银杏雌花芽分化期间内源激素、碳水化合物和矿质营养的变化. 浙江农林大学学报, 23(5):532-537.
(Luo P Y, Shi J K, Zhang W P. 2006. Changes of endogenous hormones,carbohydrate and mineral nutrition during the differentiation of female flower buds of Ginkgo biloba. Journal of Zhejiang A＆F University, 23(5):532-537. [in Chinese])
彭向永. 2017.雌、雄蒿柳花芽分化机制及性别决定基因挖掘.北京:中国林业科学研究院博士论文.
(Peng X Y. 2017. Mechanism of floral bud differentiation and discovery of sex determination genes in male and female Salix viminalis. Beijing:PhD thesis of Chinese Academy of Forestry. [in Chinese])
汪俏梅, 曾广文. 1997. 高等植物性别分化的诱导信号. 植物生理学通讯, 33(2):147-151.
(Wang Q M, Zeng G W. 1997. Inducing signal of sex differentiation in higher plants.Plant Physiology Communications, 33(2):147-151. [in Chinese])
史继孔, 张万萍,樊卫国, 等. 1999. 银杏雌花芽分化过程中内源激素含量的变化. 园艺学报, 26(3):194-195.
(Shi J K, Zhang W P, Fan W G, et al. 1999.Changes in endogenous hormones during the differentiation of female flower bud of Ginkgo (Ginkgo biloba L.).Acta Horticulturae Sinica, 26(3):194-195. [in Chinese])
张上隆, 阮勇凌, 储可铭, 等. 1990. 温州蜜桔花芽分化内源玉米素和赤霉素的变化. 园艺学报, 17(4):270-274.
(Zhang S L, Ruan Y L, Chu K M, et al. 1990. Changes of endogenous zeatin and gibberellic acid in Citrus satsuma during the period of flower bud formation.Acta Horticulturae Sinica, 17(4):270-274. [in Chinese])
张万萍, 史继孔. 2004. 银杏雄花芽分化期间内源激素、碳水化合物和矿质营养含量的变化. 林业科学, 40(2):51-54.
(Zhang W P, Shi J K. 2004. Changes of endogenous hormones,carbohydrate and mineral nutritions during the differentiation of male flower buds in Ginkgo biloba.Scientia Silvae Sinicae, 40(2):51-54. [in Chinese])
钟晓红, 罗先实, 陈爱华. 1990. 李花芽分化与体内主要代谢产物含量的关系. 湖南农业大学学报, 25(1):31-35.
(Zhong X H, Luo X S, Chen A H. 1990. A study on Nai Plum's flower bud differentiation and its major content of metabolic production. Journal of Hunan Agricultural University, 25(1):31-35. [in Chinese])
周华. 2015. 基于转录组比较的牡丹开花时间基因挖掘. 北京:北京林业大学博士论文.
(Zhou H. 2015. Discovery of gene associated with flowering time in tree peonies based on transciptome comparison. Beijing:PhD thesis of Beijing Forestry University. [in Chinese])
周学明, 马焕普, 王凤珍, 等. 1988. 不同时期苹果花芽和叶芽中内源赤霉素、脱落酸和细胞分列式活性的变化. 中国农业科学, 21(3):41-45.
(Zhou X M, Ma H P, Wang F Z, et al. 1988. Variation of gibberellins, cytokinins and abscisic acid in vegetative and flower buds in various periods of apple tree.Scientia Agricultura Sinica, 21(3):41-45. [in Chinese])
朱振家, 姜成英, 史艳虎, 等. 2015. 油橄榄成花诱导与花芽分化期间侧芽内源激素含量变化. 林业科学, 51(11):32-39.
(Zhu Z J, Jiang C Y, Shi Y H, et al. 2015. Variations of Endogenous Hormones in Lateral Buds of Olive Trees(Olea europaea) during floral induction and flower-bud differentiation. Scientia Silvae Sinicae, 51(11):32-39. [in Chinese])
Amasino R M. 2010. Seasonal and developmental timing of flowering. The Plant Journal, 61(6):1001-1013.
Andreini L, Bartolini S, Guivarc'h A, et al. 2008. Histological and immunohistochemical studies on flower induction in the olive tree (Olea europaea L.). Plant Biology, 10(5):588-595.
Andres F, Coupland G. 2012. The genetics basis of flowering responses to seasonal cues. Nature Reviews Genetics, 13(9):627-639.
Applewhite P B, Kaur-Sawhney R, Galston A W. 2000. A role for spermidine in the bloting and flowering of Arabidopsis. Physiologia Plantarum, 108(3):314-320.
Chandler J W. 2011.The hormonal regulation of flower development. Journal of Plant Growth Regulation, 30(2):242-254.
Chutinanthakun T, Sekozawa Y, Sugaya S, et al. 2015. Effect of bending and the joint tree training system on the expression levels of GA_3^-, and GA_2^- oxidases, during flower-bud development in ‘Kiyo' Japanese plum.Scientia Horticulturae, 193(6):308-315.
Costa G, Barakli R, Bagni N. 1986.Effect of putreseinon fruiting performance of apple(cv. Hi Early).Acta Horticulturae, 179(1):355-361.
Cui Z, Zhou B, Zhang Z, et al. 2013. Abscisic acid promotes flowering and enhance LcAP1 expression in Litchi chinensis Sonn. South African Journal of Botany. 88(9):76-79.
D'Aloia M, Bonhomme D, Bouché F, et al. 2011. Cytokinin promotes flowering of Arabidopisis via transcriptional activation of the FT paralogue TSF. The Plant Journal, 65(6):972-979.
Davis S J. 2009.Integrating hormones into the floral-transition pathway of Arabidopsis thaliana. Plant Cell and Environment, 32(9):1201-1210.
De M A, Tóth R, Coupland G. 2010. Plant development goes like clockwork. Trends in genetics, 26(7):296-306.
Durand B, Durand R. 1991. Sex determination and reproductive organ differentiation in Mercurialis.Plant Science, 80(1):49-65.
Falasca G, Franceschetti M, Bagni N, et al. 2010. Polyamine biosynthesis and control of the development of functional pollen in kiwifruit. Plant Physiology Biochemistry, 48(7):565-573.
Fu X, Harberd N P. 2001. Expression of Arabidopsis GAIin transgenic rice represses multiple gibberellin responses. Plant Cell, 13(8):1791-1802.
Fukazawa J, Teramura H, Murakoshi S et al. 2014. DELLAS function as coactivators of GAI-associated factors in regulation of gibberellin homeostasis and signaling in Arabidopsis. Plant Cell, 26(7):2920-2938.
Fujioka S, Yamane H, Spray C R, et al. 1988. Qualitative and quantitative analyses of gibberellins in vegetative shoots of normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 seedlings of Zea mays L. Plant Physiology, 88(4):1367-1372.
Gao X, Walworth A E, MacKie C, et al. 2016. Overexpression of blueberry FLOWERING LOCUS T is associated with changes in the expression of phytohormone-related genes in blueberry plants. Horticulture Research, 3:16053.DOI:https://doi.org/10.1038/hortres.2016.53.
Gordon S P, Chickarmane V S, Ohno C, et al. 2009. Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem. Proceedings of the National Academy of Sciences of the United States of America, 106(38):16529-16534.
Greenboim-Wainberg Y, Maymon I, Borochov R, et al. 2005.Cross talk between gibberellin and cytokinin:the Arabidopsis GA response inhibitor SPINDLY plays a positive role in cytokinin signaling. Plant Cell, 17(1):92-102.
Imamura T, Fujita K, Tasaki K, et al. 2015. Characterization of spermidine synthase and spermine synthase—The polyamine-synthetic enzymes that induce early flowering in Gentiana triflora. Biochemical & Biophysical Research Communication, 463(4):781-786.
Lee J, Lee I. 2010. Regulation and function of SOC1, a flowering pathway integrator. Journal of Experimental Botany, 61(9):2247-2254.
Li Y M, Zhang D, Xing L B, et al. 2016. Effect of exogenous 6-benzylaminopurine (6-BA) on branch type, floral induction and initiation, and related gene expression in ‘Fuji’ apple (Malus domestica Borkh). Plant Growth Regulation, 79(1):65-70.
Lovatt C J. 1991. Stress alters ammonia and arginine metabolism//Flores H E, Arteca R N Shannon J C.Polyamines and ethylene:biochemistry, physiology, and interactions, Maryland:American Society of Plant Physiologists, 166-179.
Martin-Tanguy J. 2001. Metabolism and function of polyamines in plants:recent development (new approaches). Plant Growth Regul, 34(1):135-148.
Nakagawa M, Honsho C, Kanzaki S, et al. 2012. Isolation and expression analysis of FLOWERING LOCUS T-like and gibberellin metabolism genes in biennial-bearing mango trees. Scientia Horticulturae, 139:108-117.
Pan X Q, Welti R, Wang X M. 2008. Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry. Phytochemistry, 69(8):1773-1781.
Pan X Q, Welti R, Wang X M. 2010. Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry. Nature Protocols, 5(6):986-992.
Quinet M, Bataille G, Motyka V, et al. 2015. Hormonal regulation of floral organ development in the tomato (Solanum lycopersicum L.) mutant stamenless.Plant Organ Growth Symposium (Gent, Belgium, du 10/03/2015 au 12/03/2015).
Rossi G, Scaglione G, Longo C P, et al. 1990. Sexual differentiation in Asparagus officinalis L. Hormonal content and peroxidase isoenzymes in female and male plants. Sexual Plant Reproduction, 3(4):236-369.
Sakamoto T, Kamiya N, Ueguchi-Tanaka M,et al. 2001. KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem. Genes & Development, 15(5):581-590.
Smalle J, Kurepa J, Yang P, et al. 2003. The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling. Plant Cell, 15(4):965-980.
Sood S, Nagar P K. 2004. Changes in endogenous polyamines during flower development in two diverse species of rose. Plant Growth Regulation, 44(2):117-123.
Stokes T S, Croker S J, Hanke D E. 2003.Developing inflorescences of male and female Rumex acetosa L. show differences in gibberellin content. Journal of Plant Growth Regulation, 22(3):228-239.
Tsai A Y, Gazzarrini S. 2014. Trehalose-6-phosphate and SnRK1 kinases in plant development and signaling:the emerging picture. Frontiers in Plant Science, 5:119.DOI:10.3389/fpls.2014.00119.
Tseng T S, Salomé P A, Mc Clung C R, et al. 2004.SPINDLY and GIGANTEA interact and act in Arabidopsis thaliana pathways involved in light responses, flowering, and rhythms in cotyledon movements. Plant Cell, 16(6):1550-1563.
Ulger S, Sonmez S, Karkacier M, et al. 2004. Determination of endogenous hormones, sugars and mineral nutrition levels during the induction, initiation and differentiation stage and their effects on flower formation in olive. Plant Growth Regulation, 42(1):89-95.
Wen X, Luo K, Xiao S, et al. 2016. Qualitative analysis of chemical constituents in traditional Chinese medicine analogous formula cheng-Qi decoctions by liquid chromatography-mass spectrometry. Biomedical Chromatography, 30(3):301-311.
Wilkie J D, Sedgley M, Olesen T. 2008. Regulation of floral initiation in horticulturae trees. Journal of Experimental Botany, 59(12):3215-3228.
Xing L B, Zhang D, Li Y M, et al. 2015. Transcription profiles reveal sugar and hormone signaling pathways mediating flower induction induction in apple (Malus domestica Borkh.). Plant Cell Physiology, 56(10):2052-2068.
Zhai F F, Mao J M, Liu J X,et al. 2016. Male and female subpopulations of Salix viminalis present high genetic diversity and high long-term migration rates between them. Frontiers in Plant Science, 7:330.
Zhao Y, Liang H Y, Li L, et al. 2015. Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways. Frontiers in Plant Science, 6:467:DOI:10.3389/fpls.2015.00467.

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Variations of Endogenous Hormones and Polymines during Flowering Process in Male and Female Salix viminalis

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