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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (10): 48-53.doi: 10.11707/j.1001-7488.20131008

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Biological Characteristics of Pollen Germination of ‘Taishanhong’ Pomegranate

Yang Shangshang1,2, Yuan Zhaohe1, Li Yun1, Li Qin1, Yin Yanlei1, Feng Lijuan1, Zhao Xueqing1   

  1. 1. Shandong Institute of Pomology Tai'an 271000;
    2. College of Forestry, Shandong Agricultural University Tai'an 271018
  • Received:2012-10-26 Revised:2013-02-23 Online:2013-10-25 Published:2013-11-05

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Abstract:

The cultivar, ‘Taishanhong’, of pomegranate (Punica granatum) was used as experimental materials to investigate the course of flowering and pollen germination to find the optimal culture conditions of pollen germination and to study the effects of plant growth regulators on pollen germination and pollen tube growth. The results showed that there were three morphological phases during flowering periods. The anther released pollens the next day from 8:00 to 17:00 (22-30 ℃,relative humidity 25%-40%) when flowers were completely open. The pollen dispersion period usually took 1-2 days. The optimal culture conditions of pollen germination were with 10% sucrose,0.005% H3BO3 and pH 7.0. The time of pollen germination was 3 h. Low concentration 6-BA (1-10 mg·L-1) and 2,4-D (1-5 mg·L-1) promoted the pollen germination and tube growth. However, high concentrations of the hormones expressed inhibition effects on the pollen germination and tube growth. The optimal concentration for pollen germination pollen and tube growth was 10 mg·L-1(6-BA) and 5 mg·L-1(2,4-D). In the contrast, NAA (1-30 mg·L-1) and PP333(50-800 mg·L-1) had some inhibitory effects on the pollen germination and tube growth of pomegranate. The inhibitory effects strengthened with concentration increasing.

Key words: Punica granatum, pollen germination, plant growth regulators

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