3种竹类植物杂种的分子鉴定

doi:10.11707/j.1001-7488.20090306

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (3): 29-34.doi: 10.11707/j.1001-7488.20090306

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Identification of the Hybrid Bamboo F1 by SSR Markers

Lu Jiangjie¹,Yoshinaga Katsuhiko²,Fang Wei¹,Tang Dingqin ¹

(１．Key Laboratory for Modern Silvicultural Technology of Zhejiang Province Zhejiang Forestry University Linan 311300；2.World Bamboo Research Center Fukuoka 824-0076)

Received:2008-11-12 Revised:1990-01-01 Online:2009-03-25 Published:2009-03-25

Abstract

Abstract:

Abstract: Three hybrid bamboo candidates (Pleioblastus simonii × Phyllostachys violascens, Sasa tokugawana × S. borealisand Sinobambus a tootsik × Pl. distichus) were identified using 10 SSR markers developed from GSS of Ph. pubescens in GenBank which were used for amplifying the cross species. DNA fingerprinting of the 3 hybrid bamboo candidates showed complementary pattern from ♀ and ♂ parents at PBM014, PBM025 and (or) PBM018 locus. The sequencing and alignment of fragment DNA bands at the mentioned\|above loci approved homology between the hybrid bamboo candidate and their parents, indicating the validity of hybrid bamboo results. This is the first report for identification of bamboo hybrid using DNA markers.

Key words: Key words: hybrid bamboo, SSR, identification

Lu Jiangjie;Yoshinaga Katsuhiko;Fang Wei;Tang Dingqin . Identification of the Hybrid Bamboo F1 by SSR Markers[J]. Scientia Silvae Sinicae, 2009, 12(3): 29-34.

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Identification of the Hybrid Bamboo F1 by SSR Markers

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