Abstract:

Abstract： Embryogenic callus was induced and plant regeneration had been achieved by culturing spikelets and embryo explants of Bambusa multiplex. Basal media，phytohormones and their concentration were screened，and the results showed that NB and N6 basal medium supplemented with 4 mg·L^-1 2，4dichlorophenoxyacetic acid (2，4-D) were relatively better for callus induction. A 87.30% spikelets and a 76.27% embryos were induced to generate callus on NB medium supplemented with 500 mg·L^-1 proline，500 mg·L^-1 glutamine，300 mg·L^-1 hydrolyzed casein，30 g·L^-1sucrose，8 g·L^-1 carrageenan and 4 mg·L^-1 2，4-D in 20 days. Murashige and Skoog (MS) basal medium was effective for embryoids' predifferentiation and germination. After a 7 d predifferentiation on MS supplemented with 30 g·L^-1 sucrose，10 g·L^-1 carrageenan and 4 mg·L^-1 kinetin (KT) and a 14 d auxinfree germination culture on MS，only a few spikelet calli germinated； whereas embryo calli regenerated by up to 80.0％. Around 8% plantlets were albinal，and mosaic plantlets were occasionally found. Optimal predifferentiation medium for embryo embryoids was MS supplemented with 3 mg·L^-1 6benzylaminopurine (6-BA) and 3 mg·L^-1 KT. MS supplemented with 2 mg·L^-1 naphthaleneacetic acid (NAA) was effective for the plantlet rooting. Rooted plantlets transferred to soil with over 70% success.

Key words: Key words： Bambusa multiplex, callus induction, plant regeneration, media