Abstract:

In this paper, the genetic relationship among 49 female Ginkgo biloba germplasms originating from Japan, France and China was studied using 64 Eco RI/MseI primer combinations (Mse I primer is the fluorescent marker). Seven primers, that were able to produce high polymorphic and clear bands, were screened, and with them total 1 219 bands and 132 specific loci (including 11 absent bands and 121 monomorphic bands) were produced, of which 1 200 bands were polymorphic, and accounted for 98.44% of the total. The identification percent age of each primer combination was 100%. The similarity coefficient (SC) of these germplasms ranged from 0.39 to 0.83, along with different genetic variations. When SC was set at 0.51, the studied germplasms could be divided into two groups. To be interesting, the some from the same origin were not assigned to the same group. Most female germplasms from Shandong Province were classified into one group, indicating their remarkably genetic distinction and distant relationship from others. Compared with ‘Yeziyinxing', ‘Haiyang' is more primitive. Based on the comprehensive analysis on female germplasms' specific loci, similarity coefficients and cluster results, 16 germplasms, i.e, ‘Pendula' from France, ‘Huangjinwan' from Japan, ‘Panxian 1', ‘Lingchuan', ‘Anlu', ‘Dongan 1', ‘Xishandafoshou', ‘Rongcheng', ‘Juxian', ‘Tancheng', ‘Taian 1', ‘Taian 2', ‘Taian 3', ‘Laoshan', ‘Haiyang' and ‘Lingyan' from China, were recognized relatively more important among the 49 female G. biloba germplasms.

Key words: Ginkgo biloba, female germplasm, genetic relationship, polymorphism, AFLP