Abstract:

To investigate the gene expression and to separate clones which are important genes related to oil synthesis and so on during transforming peak of seed oil in Camellia oleifera, cDNA library was constructed using the nearly matured seeds of two clones, I.e. XL-1 and XL-4, and 2 327 clones were randomly selected for sequencing on the 3′-end to construct the EST library, and 1 979 sequences which are unabridged and without X or N were performed homology comparison with nucleic acid NR database. 763 cDNA sequences were highly homological to cDNA sequences of other plant species in database of NCBI, 266 kinds of genes have been identified and 1 216 clones of cDNA were unknown function of gene sequences. In the cDNA library of seeds of C. oleifera, different genes expressed different abundance. The genes related to storage protein, to expression and regulation, to resistance, to seed maturation and to embryo development were expressed in high abundance, while the genes related to metabolism of fatty acids etc. were expressed in medium abundance. The other genes responsible for many fragments and many genes unknown functions were expressed in different extent. The number and trends of expressed genes are suitable for the phase of C. oleifera seeds near to maturation.

Key words: Camellia oleifera, cDNA, EST, Library, Expressed genes