Abstract:

Brown spot needle blight fungus (Lecanosticta acicola) could produce pathogenic toxin. A combination use of silica gel column chromatography, reversed phase silica gel column chromatography and HPLC led to the isolation of a phytotoxic compound, named LA-I, from the crude toxins produced by Lecanosticta acicola cultured in PD medium. LA-I was colorless oil. UV scanning results indicated the maximum absorptive wave length of LA-I was 207 nm. Under 365 nm and 254 nm, LA-I had no or little UV absorption. The MW and MF of this toxin was determined by FAB^-MS and HRFAB^-MS as 162 and {C_{61051HNMR spectrum showed —OCH(CH313CMNR spectrum indicated not only the —OCH(CH3-MS, HRFAB^-MS,¹HNMR,¹³CNMR data, the structure of LA-I could be deduced as COOHCH(CH33}

Key words: Lecanosticta acicola, mycotoxin, &alpha, &alpha, &prime, di-propanacid aether