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Scientia Silvae Sinicae ›› 2004, Vol. 40 ›› Issue (5): 138-142.doi: 10.11707/j.1001-7488.20040523

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Selection of Bacillus thuringiensis Strain against the Longhorned Beetles and Preliminary Characterization of Its Insecticidal Gene

Chen Jun,Du Mengfang,Yin Xinming,Wang Xuepin,Lu Mengzhu   

  1. Research Institute of Forestry, CAF Key Laboratory of Forest Silviculture of the State Forestry Administration Beijing100091；College of Plant Protection, Henan Agricultural University Zhengzhou450002
  • Received:2004-03-09 Revised:1900-01-01 Online:2004-09-25 Published:2004-09-25

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Abstract:

A Bacillus thuringiensis strain Bt886 toxic to Longhorned Beetle (Anoplophora glabripennis) larvae were isolated from dead body of Tribolium macleby. The larvae fed with Bt886 exhibited high mortality (60%) and significant delay in growth. The shape of its crystal and spectrum of affected insects indicated that Bt886 could harbor cry3 like genes. A pair of primers was designed based on the conservative region of cry3 genes and a DNA fragment was successfully obtained by amplification. The fragment was further cloned into pGEM-T and the sequence analysis showed highly homologous to cry3 Aa1 gene with only one nucleotide difference. The fragment was located on plasmid of Bt886 by Southern analysis.

Key words: Longhorned Beetle, Bacillus thuringiensis, cry 3A gene, Southern analysis