苏云金杆菌对松墨天牛幼虫的致死作用及其生物学特性

doi:10.11707/j.1001-7488.20100423

Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (4): 151-155.doi: 10.11707/j.1001-7488.20100423

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Virulence of Bacillus thuringiensis to Monochamus alternatus and Its biological Characteristics

Xu Huachao¹,Xu Jinhua^1,2,Zhang Liqin¹,Lin Haiping¹,Yang Ping³,Cheng Jianming²

1.School of Forestry and Bio-Technology，Zhejiang Forestry University Linan 311300； 2. Jiangjia Forestry Central Station， Chun’an Forestry Bureau Chunan 311712；3. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province Linan 311300

Received:2009-04-30 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25

Abstract

Abstract:

The virulence of 17 Bacillus thuringiensis strains against 2-3 instar larvae of Monochamus alternatus were tested in indoor. The results showed that two strains (RBT-200701, RBT-200702) exhibited high poisonous activity, and the caused corrected mortality of 2-3 instar M. alternatus larvae were all more than 70%. A positive correlation was found between the corrected mortality and the concentration of the mixtures of spores and parasporal crystal (125-2 000 μg·g^-1). The insecticidal effect increased with treatment time (0-20 d). The morphological changes of the two strains at various developmental stages were examined with microscopy on the cell smear. The optimal fermentation condition of strains was 30 ℃, pH7.2, 200 r·min^-1, for 36 h. The two strains had the same protein spectrum after SDS-PAGE analysis.

Key words: Bacillus thuringiensis, Monochamus alternatus, biological control, pine wilt disease

Xu Huachao;Xu Jinhua;Zhang Liqin;Lin Haiping;Yang Ping;Cheng Jianming. Virulence of Bacillus thuringiensis to Monochamus alternatus and Its biological Characteristics[J]. Scientia Silvae Sinicae, 2010, 46(4): 151-155.

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Virulence of Bacillus thuringiensis to Monochamus alternatus and Its biological Characteristics

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