Tangtsinia nanchuanica is a kind of rare plants, a class Ⅱ protected species in China. Its wild individuals have been hardly found. In this paper, the eco-physiological characteristics of Tangtsinia nanchuanica and its relative Cephalanthera falcata were compared. Using the LI COR6400 Portable Photosynthesis System, we measured some eco-physiological indicators of the two plants. Except the indicators such as stomatal conductivity (Con), water use efficiency (WUE) and internal CO2 concentration(Ci), Tangtsinia nanchuanica and Cephalanthera falcata had not significant differences in net photosynthetic rate(Pn), transpiration rate(E) and other indicators. The Pn, dark respiration rate(Rd),E, Con, Ci, Light compensation point (LCP) , light saturation point (LSP) , solar energy use efficiency (SUE) and WUE of Tangtsinia nanchuanica were 6.16(±0.4) μmol·m-2s-1,0.47(±0.04) μmol·m-2s-1,2.7(±0.2) mmol·m-2s-1, 0.114(±0.02) mol·m-2s-1, 206(±29) μmol·mol-1, 10 μmol photons·m-2s-1, 200 μmol photons·m-2s-1, 9.6(±0.9)%, 3.1(±0.2) mmol CO2·mol-1 H2O respectively. Accordingly, the Pn,Rd, E, Con, Ci, LCP, LSP, SUE and WUE of Cephalanthera falcata were 5.8 (±1.1) μmol·m-2s-1, 0.45(±0.06) μmol·m-2s-1, 2.6(±0.3) mmol·m-2s-1, 0.101(±0.03) mol·m-2s-1, 173(±51) μmol·mol-1, 10 μmol photons·m-2s-1, 200 μmol photons·m-2s-1, 9.1(±1.1)%, 2.7(±0.7) mmol CO2·mol-1 H2O. In the greenhouse, the high Pn values of the orchids were observed from 9:00 to 13:00 and the midday photosynthetic depression was not observed on sunny days in summer. According to the light response of Pn, Tangtsinia nanchuanica and Cephalanthera falcata were both shade tolerant plant but could tolerate strong lights less than 1 000 μmol photons·m-2s-1. Based on the temperature response of Pn, the favorable temperature for the two orchids was 15~25℃. By the stepwise multiple regression, it was found that E, WUE, Con, Ci, air temperature and PAR were the most dominant factors affecting the Pn of Tangtsinia nanchuanica, and the Con, Ci, air CO2 concentration, E, PAR, relative humidity and SUE were the most dominant factors affecting the Pn of Cephalanthera falcata.