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林业科学 ›› 2004, Vol. 40 ›› Issue (6): 45-49.doi: 10.11707/j.1001-7488.20040608

• 论文及研究报告 • 上一篇    下一篇

内源激素含量与银杏叶中类黄酮含量的关系

程水源 王燕 李俊凯 费永俊 朱桂才   

  1. 长江大学,荆州434023
  • 收稿日期:2003-01-22 修回日期:1900-01-01 出版日期:2004-11-25 发布日期:2004-11-25

Study on the Relationship between the Endogenous Hormones and Flavonoids in Ginkgo biloba Leaf

Cheng Shuiyuan,Wang Yan,Li Junkai,Fei Yongjun,Zhu Guicai   

  1. Yangtze University Jingzhou 434023
  • Received:2003-01-22 Revised:1900-01-01 Online:2004-11-25 Published:2004-11-25

摘要:

对银杏叶丛枝叶中内源激素和类黄酮含量进行全周期测定。结果表明:早期脱落酸(ABA)含量低,但在7月初有一次绝对值较低的含量峰,8月底后含量迅速上升,在11月初达到含量最大值,含量峰与叶中类黄酮峰同步;乙烯(ETH)在7月初和9月底形成2次高峰,第2次峰值较大,第1次峰与叶中类黄酮峰平行,第2次峰超前;细胞激动素(CTKs)含量早期高,后波动次数较多,玉米素(Zrs)与CTKs含量变化曲线极显著相关;赤霉素(GA)含量早期较高,中后期含量较低,在7月中旬、9月底2次达到含量低谷;吲哚乙酸(IAA)含量在5月中旬、8月中旬、9月底出现3次含量高峰,第1次峰值最大;较高的ETH、ABA含量,较大的CTKs IAA值,较低的GA含量有利于叶类黄酮合成;在最低的IAA/ABA、IAA/ETH、CTKs/ABA、CTKs/ETH、GA/ABA、GA/ABA、GA/ETH值时,相应地在银杏叶中有类黄酮合成高峰。讨论了内源激素与叶中类黄酮之间的关系。

关键词: 银杏, 叶, 内源激素, 类黄酮

Abstract:

The contents of the flavonoids and endogenous hormones of leaves in Ginkgo biloba phyllome branches were determined during all stages. The results indicated that the abscisic acid (ABA) content was low in the early stage, and got its lower peak in the early July, and rose quickly from the middle of Aug., and reached its highest value in the early Nov., which was consistent with the flavonoids content peak. Typical two-peak curve belonged to the change of ethylene (ETH) concentration, one rested in the early July, the other with a higher value in the late Sep. Cytokinin (CTKs) content was high in the early stage and fluctuated several times with different ranges in the middle-late stage. There was a significant relationship between CTKs and zeatin ribosides(Zrs). Gibberellin (GA) content was high in the early stage, and then went down gradually to reach its lowest value in the middle July, and lower value in the late Sep. apiece. 3-indole acetic acid (IAA) content fluctuated with a large scope, and showed three peaks during the growth and development of the leaves. Of these peaks, the first peak was the highest. It was very helpful for the improvement of the flavonoids synthesis to have higher ETH, ABA, and CTKs/IAA, and lower GA. When IAA/ABA, IAA/ETH, CTKs/ABA, CTKs/ETH, GA/ABA, GA/ETH, IAA/ABA, IAA/ETH, GA/ABA, GA/ETH values were the lowest, the peaks of flavonoids formation could be observed accordingly. The relationship between flavonoids and endogenous hormones in G. biloba leaf was discussed in this paper.

Key words: Ginkgo biloba, Leaf, Endogenous hormone, Flavonoids