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林业科学 ›› 2006, Vol. 42 ›› Issue (4): 38-43.doi: 10.11707/j.1001-7488.20060407

• 论文及研究报告 • 上一篇    下一篇

油茶种质资源遗传多样性分析

黄永芳1 陈锡沐1 庄雪影1 雷治国1 陈永忠2 彭邵峰2   

  1. 1.华南农业大学林学院,广州510642;2.湖南省林业科学院,长沙410004
  • 收稿日期:2005-03-20 修回日期:1900-01-01 出版日期:2006-04-25 发布日期:2006-04-25

Analysis of Genetic Diversity in Camellia oleifera Germplasms

Huang Yongfang1,Chen Ximu1,Zhuang Xueying1,Lei Zhiguo1,Chen Yongzhong2,Peng Shaofeng2   

  1. 1. Forestry College of South China Agricultural University Guangzhou 510642;2. Hunan Academy of Forestry Changsha 410004
  • Received:2005-03-20 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

摘要:

利用RAPD标记技术分析90份油茶种质的遗传多样性,探讨无性系间的亲缘关系,以及抗病性、果油率和产油量3个数量性状与谱带的相关性。结果表明:RAPD扩增得到593条谱带,多态性谱带564条,多态比率达95.11%,特异性及特异性缺失谱带32条,占5.32%,无性系间存在丰富的遗传多样性;广西软枝油茶和广东阳春油茶均聚在同一亚类,从分子水平说明了阳春油茶和软枝油茶的亲缘关系比较近,可能是同一个农家品种;与抗病性、果油率和产油量3个数量性状显著相关谱带分别为13.1%、25.2%和34.5%,这些与抗病性、果油率和产油量数量性状相关谱带的确定,可为选育丰产、抗病、优质的油茶品种提供参考。

关键词: 油茶, RAPD, 遗传多样性, 种质资源

Abstract:

The genetic diversity of 90 Camellia oleifera clones was investigated and the relationship between these clones was analyzed using RAPD technique. Correlations between three quantitative traits (disease resistance, the rate of oil and oil yield) and molecular markers were also analyzed. The results are as follows: The total number of amplified fragments is 593, among which there are 564 polymorphic bands, the percentage of polymorphic bands is 95.11%. There are 32 distinctive bands(including missing distinctive bands),accounting for 5.32% in total bands. Abundant genetic diversity exists among C. oleifera clones.‘Yangchun’ and ‘Ruanzhi’ of C. oleifera aggregated in the same subgroup, indicating that they have close relationship, and could be the same agronomic cultivar at molecular level. Bands related to disease resistance, the rate of oil and oil yield account for 13.1%, 25.2% and 34.5% respectively, and could be used as reference in selecting resistant C. oleifera cultivars with both high quality and high yield.

Key words: Camellia oleifera, RAPD, genetic diversity, germplasm resources