关键词: 墨西哥落羽杉, 离体培养, 植株再生

Abstract:

Young seedlings developing from the seeds were cultured as the initiation explants to investigate the micropropagation of Taxodium mucronatum. Cultured young seedlings on DCR medium supplemented with 0.5 mg·L^-1 BA and 0.2 mg·L^-1 IBA was the best way of inducement and differentiation,with inducement rate higher than 80%,mean number of induced buds around 2～5. For elongation of the induced buds,the suitable culture medium was DCR+Gln 500 mg·L^-1 . The medium of 3/4 DCR+0.05% AC was used for rooting,and the rooting rate was more than 75%. Using hormone to induce roots could produce mass of callus on the end top of cuttings and it was not benefit to the plantlets survival after transplanting. In addition,it was easy for the plantlets with long internode and vigorous stem to induce root system. When the roots developed to 2～4 cm long,the plantlet could be transplanted after 3～7 days acclimation.

Key words: Taxodium mucronatum, tissue culture, in vitro culture, plantlet regeneration