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林业科学 ›› 2007, Vol. 43 ›› Issue (07): 30-34.doi: 10.11707/j.1001-7488.20070706

• 论文及研究报告 • 上一篇    下一篇

曼地亚红豆杉的组织培养快繁技术

马均 马明东   

  1. 四川农业大学都江堰分校,都江堰611830
  • 收稿日期:2006-09-01 修回日期:1900-01-01 出版日期:2007-07-25 发布日期:2007-07-25

Tissue Culture Technique of Taxus media ‘Hecksii’

Ma Jun,Ma Mingdong   

  1. Dujiangyan Campus of Sichuan Agriculture University Dujiangyan 611830
  • Received:2006-09-01 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

摘要:

以曼地亚红豆杉扦插苗当年生枝的带芽茎段为外植体,进行腋芽增殖再生植株育苗试验。结果表明:MS+6-BA0.05mg·L-1+NAA0.5mg·L-1是曼地亚红豆杉腋芽增殖启动培养的适宜培养基,启动率89.55%。6-BA对芽的启动是必需,但浓度稍高启动率便极显著下降。生长调节物质对芽的启动有利但抑制芽的生长,不添加生长调节物质的MS(有机物加倍)培养基是曼地亚红豆杉侧芽继代培养的适宜培养基,有效芽率为72.73%,平均芽长3.3cm。曼地亚红豆杉组培生根不定根的诱导比较困难,生根率最高27.78%。不定根的发生需要在植物体内建立一个最佳的细胞分裂素和生长素的平衡,而不仅是需要生长素。用IBA1000mg·L-1作生根促进剂处理30s进行瓶外生根,生根率达到58.33%,是进行曼地亚红豆杉组培无根苗生根诱导的适宜处理方式。

关键词: 曼地亚红豆杉, 组培, 腋芽增殖, 植株再生

Abstract:

Young stem section with buds of cutting plantlets of Taxus media ‘Hicksii’ was used as explants for armpit bud multiplication plantlet regeneration experiment. The results showed, MS+6-BA 0.05 mg·L-1 +NAA 0.5 mg·L-1 is the optimal culture medium for startup culture, the startup rate is 89.55%. 6-BA is indispensable for startup, but the concentration of it should be low. The growth regulators were beneficial for the startup of the buds, but harmful for the growth of it. MS (double organic matter) culture medium with no growth regulators was suitable for buds to grow on. The valid buds rate is 72.73%; mean length of buds is 3.3 cm. The induction of adventitious roots needed to establish a suitable balance of auxin and cytokinin in the plantlet. Retting cuttings in IBA liquor (1 000 mg·L-1) 30 s was a fit means for root induction; the rooting rate is 58.33%.

Key words: Taxus media &lsquo, Hecksii&rsquo, tissue culture, armpit bud multiplication, plantlet regeneration