大花黄牡丹遗传多样性的SRAP分析

doi:10.11707/j.1001-7488.20120112

林业科学 ›› 2012, Vol. 48 ›› Issue (1): 70-76.doi: 10.11707/j.1001-7488.20120112

大花黄牡丹遗传多样性的SRAP分析

唐琴¹, 曾秀丽^2,3, 廖明安¹, 潘光堂³, 扎西², 龚君华², 次仁卓嘎²

1. 四川农业大学园艺学院雅安 625014;2. 西藏自治区农牧科学院蔬菜研究所拉萨 850032;3. 四川农业大学玉米研究所雅安 625014

收稿日期:2011-03-11 修回日期:2011-11-22 出版日期:2012-01-25 发布日期:2012-01-25
通讯作者: 廖明安

SRAP Analysis of Genetic Diversity of Paeonia ludlowii in Tibet

Tang Qin¹, Zeng Xiuli^2,3, Liao Ming'an¹, Pan Guangtang³, Zha Xi², Gong Junhua², Ciren Zhuoga²

1. College of Horticulture, Sichuan Agricultural University Ya'an 625014;2. Institute of Vegetables, Tibet Academy of Agricultural and Animal Husbandry Sciences Lahsa 850032;3. Institute of Maize, Sichuan Agricultural University Ya'an 625014

Received:2011-03-11 Revised:2011-11-22 Online:2012-01-25 Published:2012-01-25

摘要/Abstract

摘要：

应用SRAP标记对西藏特有植物大花黄牡丹的遗传多样性进行研究。用16对引物从5个自然居群79个单株中共检测到396个有效位点,其中多态性位点357个。在物种水平上,多态位点百分率(P_pl)为90.15%,Shannon表型多样性指数(Η_sp)平均为0.252 1; 居群水平上的P_pl为31.82%,Shannon表型多样性指数(H_o)为0.069 4~0.342 8,平均值(Η_pop)为0.130 7。上述遗传参数表明,大花黄牡丹具有丰富的物种遗传多样性,5个居群中自然居群C的遗传多样性最高(P_pl=82.32%,H_o=0.342 8)。据AMOVA分析结果,总的变异中有41.58%的变异存在于居群间,58.42%的变异存在于居群内,居群分化较显著(Φ_ST=0.415 8,P<0.001),由POPGENE1.32得到的居群间遗传分化系数G_ST(0.430 9)和Shannon表型多样性指数计算的居群间遗传多样性所占比例(0.481 6)也表明了类似的遗传结构。Mantel检测表明地理距离和Nei's遗传距离间相关不显著(P>0.05)。利用NTSYS_PC(2.1)软件构建大花黄牡丹5个居群79个个体的UPGMA聚类图,遗传相似系数变幅在0.47~0.99,大多数居群内的个体表现出较为密切的亲缘关系(如居群B,D,E),但也有一些居群的个体未聚在一起(如居群C)。依据大花黄牡丹居群遗传变异特点,初步探讨其保护和利用策略。

关键词: 大花黄牡丹, SRAP, 居群, 遗传多样性, 遗传分化

Abstract:

Sequence Related Amplified Polymorphism (SRAP) analysis was applied to study the genetic diversity of Paeonia ludlowii, a species only distributed in Tibet of China. Sixteen pairs of SRAP primers were used to evaluate the genetic diversity of 79 samples from five natural populations. A total of 396 valid loci were obtained, of which 357 were exhibited polymorphism. The percentage of polymorphic loci (P_pl) and the mean diversity estimated with Shannon's index of phenotypic diversity(Η_sp) at species level were 90.15% and 0.252 1, respectively.At population level, P_pl was 31.82% and Shannon's index (H_o) ranged from 0.069 4 to 0.342 8, with an average value(Η_pop) of 0.130 7. Among the five populations, population C was especially more diverse(P_pl=82.32%,H_o=0.342 8). Those results indicated that there was a relatively high level of intraspecific genetic diversity. Analysis of molecular variance(AMOVA) demonstrated that a high degree of genetic differentiation occurred among the populations (Φ_{ST =0.415 8,P<0.001). The proportion of genetic variation among populations was 41.58%, while the proportion within population was 58.42%. A similar analysis result was exhibited by the genetic differential index(G_ST) of 0.430 9 and the proportion of diversity among populations with Shannon's index of 0.481 6. Mantel test showed that there was no significant correlation between geographical distance and genetic distance (r=0.597 5,P=0.905 5) among populations. A dendrogram conducted by UPGMA method using NTSYS_PC(2.1) demonstrated that the range of genetic similarity coefficient was 0.47-0.99. The most of the individuals came from the same population had close genetic relationship, such as population of B, D, E,but individuals from population C could not be clustered together completely. A strategy for the conservation and utilization of P. ludlowii based on genetic diversity was proposed.}

Key words: Paeonia ludlowii, SRAP, population, genetic diversity, genetic differentiation

中图分类号:

唐琴;曾秀丽;廖明安;潘光堂;扎西;龚君华;次仁卓嘎. 大花黄牡丹遗传多样性的SRAP分析[J]. 林业科学, 2012, 48(1): 70-76.

Tang Qin;Zeng Xiuli;Liao Ming'an;Pan Guangtang;Zha Xi;Gong Junhua;Ciren Zhuoga. SRAP Analysis of Genetic Diversity of Paeonia ludlowii in Tibet[J]. Scientia Silvae Sinicae, 2012, 48(1): 70-76.

[1]	张守科, 方林鑫, 刘亚宁, 王毅, 张威, 舒金平, 张亚波, 汪阳东, 王浩杰. 茶籽象ATP合成酶基因在不同海拔选择压力下的遗传分化及结构变异[J]. 林业科学, 2019, 55(6): 65-73.
[2]	金玲, 刘明国, 董胜君, 吴月亮, 张欣. 97个山杏无性系的遗传多样性及SSR指纹图谱[J]. 林业科学, 2018, 54(7): 51-61.
[3]	包文泉, 乌云塔娜, 杜红岩, 李铁柱, 刘慧敏, 王淋, 白玉娥. 基于SSR标记的西藏光核桃群体遗传多样性和遗传结构分析[J]. 林业科学, 2018, 54(2): 30-41.
[4]	邱帅, 沈柏春, 李婷婷, 郭娟, 王霁, 孙丽娜, 陈徐平, 胡绍庆. 基于随机森林算法和SRAP分子标记的桂花品种鉴定方法[J]. 林业科学, 2018, 54(1): 32-45.
[5]	杨汉波, 张蕊, 王帮顺, 徐肇友, 陈焕伟, 周志春. 木荷优树无性系种质SSR标记的遗传多样性分析[J]. 林业科学, 2017, 53(5): 43-53.
[6]	毛秀红, 郑勇奇, 孙百友, 张元帅, 韩丛聪, 位晓, 荀守华. 基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建[J]. 林业科学, 2017, 53(10): 80-89.
[7]	冯源恒, 李火根, 杨章旗, 黄永利, 罗群凤, 张远. 广西马尾松第2代育种群体的组建[J]. 林业科学, 2017, 53(1): 54-61.
[8]	宋志姣, 杨合宇, 翁启杰, 周长品, 李发根, 李梅, 卢万鸿, 罗建中, 甘四明. 细叶桉群体的遗传多样性和受选择位点[J]. 林业科学, 2016, 52(9): 39-47.
[9]	徐永杰, 韩华柏, 王滑, 陈凌娜, 马庆国, 裴东. 大巴山区核桃实生居群的坚果表型和遗传多样性[J]. 林业科学, 2016, 52(5): 111-119.
[10]	廖柏勇, 王芳, 陈丽君, 刘明骞, 欧阳昆唏, 阙青敏, 惠文凯, 李培, 陈晓阳. 基于SRAP分子标记的苦楝种质资源遗传多样性分析[J]. 林业科学, 2016, 52(4): 48-58.
[11]	杨汉波, 张蕊, 周志春. 木荷种子园的遗传多样性和交配系统[J]. 林业科学, 2016, 52(12): 66-73.
[12]	李培, 阙青敏, 欧阳昆唏, 李俊成, 湛欣, 朱芹, 张俊杰, 邓小梅, 陈晓阳. 不同种源红椿SRAP标记的遗传多样性分析[J]. 林业科学, 2016, 52(1): 62-70.
[13]	张振, 张含国, 莫迟, 张磊. 红松转录组SSR分析及EST-SSR标记开发[J]. 林业科学, 2015, 51(8): 114-120.
[14]	梁玉琴, 张嘉嘉, 梁晋军, 李芳东, 韩卫娟, 傅建敏. 河南省柿种质资源的遗传多样性[J]. 林业科学, 2015, 51(6): 71-80.
[15]	尚帅斌, 郭俊杰, 王春胜, 赵志刚, 曾杰. 海南岛青梅天然居群表型变异[J]. 林业科学, 2015, 51(2): 154-162.

大花黄牡丹遗传多样性的SRAP分析

SRAP Analysis of Genetic Diversity of Paeonia ludlowii in Tibet

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式