美洲黑杨(Populus deltoides Marsh)×青杨(P.cathayana Rehd.)分子连锁图谱的构建*

摘要/Abstract

摘要：

利用随机扩增DNA多态性RAPD方法,在美洲黑杨(P.deltoides Marsh)×青杨(P.cathayana Rehd.)3代谱系中分析分子标记,构建出第1张美洲黑杨×青杨分子连锁图谱。共从300个10-mer随机引物中筛选出79个适合引物,检测出可供构图的分离标记180个。该图谱由20个连锁群, 110个RAPD标记组成。总图距为覆盖基因组总长度的70.35%,标记间的平均间距为17.27cM.连锁群长度在37.1~189.8,相应标记分别在3~10。本图谱为杨树抗病、虫和其它性状基因定位提供了框架结构,为实现杨树分子遗传育种迈进了最重要的一步。

关键词: 分子连锁图, RAPD, 美洲黑杨, 青杨

Abstract:

A molecular linkage map was constructed for P.deltoides Marsh × P.cathayana Rehd.based on random amplified polymorphic DNA (RAPD) markers.The mapping pedigree consists of three generations with the F₁ produced by interspecific hybridization between a P.deltoides female and a P.cathayana male.Three-hundred random 10-base RAPD primers were screened in amplification reactions using DNA isolated from parents P.deltoides, P.cathayana and their F₁ recombinant inbred population consisting of 4 lines.Of these primers, 180 revealed at least one polymorphic RAPD locus which can be used by mapping.This map identified 20 linkage groups spanning 1899.4 cM (110 RAPD markers) with an average distance of 17.27 cM between markers.Linkage groups ranged from 187.8 to 37.1 cM in length and included from 3 to 10 markers, respectively.This map should facilitate the identification of markers that "tag" genes for pest and disease resistance and other traits in poplar.This is the first genetic map for P.deltoides × P.cathayana and an important step towards the molecular breeding in poplar.

Key words: Molecular linkage map, RAPD, Populus deltoides Marsh, P. cathayana Rehd

苏晓华,张绮纹,郑先武,张香华,StephenHarrish. 美洲黑杨(Populus deltoides Marsh)×青杨(P.cathayana Rehd.)分子连锁图谱的构建^*[J]. 林业科学, 1998, 34(6): 29-37.

Xiaohua Su,Qiwen Zhang,Xianwu Zheng,Xianghua Zhang,Harrish Stephen. CONSTRUCTION OF POPULUS DELTOIDES MARSH × P.CATHAYANA REHD.MOLECULAR LINKAGE MAP[J]. Scientia Silvae Sinicae, 1998, 34(6): 29-37.

图/表 3

参考文献 21

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