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林业科学 ›› 2007, Vol. 43 ›› Issue (04): 134-136.doi: 10.11707/j.1001-7488.20070424

• 研究简报 • 上一篇    下一篇

漆酶活化处理对木材自由基变化的影响

段新芳1 曹远林2 曹永建1 周冠武1 陈永圣2 朱家琪1 赵保路2   

  1. 1.中国林业科学研究院木材工业研究所,北京100091;2.中国科学院生物物理研究所,北京100101
  • 收稿日期:2005-11-10 修回日期:1900-01-01 出版日期:2007-04-25 发布日期:2007-04-25

Changes of Intensities of Reactive Oxygen Species (ROS) Free Radicals from Laccase-Treated Woods

Duan Xinfang1,Cao Yuanlin2,Cao Yongjian1,Zhou Guanwu1,Chen Yongsheng2,Zhu Jiaqi1,Zhao Baolu2   

  1. 1.Research Institute of Wood Industry, CAF Beijing 100091; 2.Institute of Biophysics, Chinese Academy of Sciences Beijing 100101
  • Received:2005-11-10 Revised:1900-01-01 Online:2007-04-25 Published:2007-04-25

关键词: 漆酶, 思茅松, 枫香, 活性氧, 自由基

Abstract:

In order to investigate the changes of free radicals in the suspension liquid of wood treated by laccase, wood powder of Pinus kesiya var. langbianensis sapwood , heartwood and Liquidambar formosana were selected to detect the changes of free radicals after laccase treatment. The laccase treatment parameters were as follows: pH 3.47 and 6.50, 2 hours of laccase incubation, the temperature of 32 ℃ and enzyme dosage 19 U·g -1 dry wood powder. The results showed that there was a significant difference of the intensities of the reactive oxygen species (ROS) free radicals in the aqueous suspension of wood between control and samples treated by laccase. The intensities of ROS free radicals among different woods treated by laccase was marked difference. The intensities of ROS free radicals of Pinus kesiya var. langbianensis sapwood or heartwood treated by laccase were higher than that of Liquidambar formosana.

Key words: laccase, Pinus kesiya var. langbianensis, Liquidambar formosana, reactive oxygen species (ROS), free radicals