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林业科学 ›› 2002, Vol. 38 ›› Issue (5): 54-59.doi: 10.11707/j.1001-7488.20020509

• 论文及研究报告 • 上一篇    下一篇

冰冻条件下外源SA对水曲柳幼苗叶片内抗氧化酶的影响

吴楚 王政权   

  1. 东北林业大学,哈尔滨150040
  • 收稿日期:2000-07-28 修回日期:1900-01-01 出版日期:2002-09-25 发布日期:2002-09-25

EFFECTS OF EXOGENOUS SA ON ACTIVITIES OF ANTIOXIDANT ENZYMES IN THE LEAVES OF FRAXINUS MANDSHURICA SEEDLINGS UNDER FREEZING STRESS

Wu Chu,Wang Zhengquan   

  1. Northeast Forestry University Harbin150040
  • Received:2000-07-28 Revised:1900-01-01 Online:2002-09-25 Published:2002-09-25

摘要:

分析了冰冻胁迫下外源水杨酸(SA ,5mmol·L-1 和10mmol·L-1 )对水曲柳幼苗叶片内抗氧化酶的影响。SOD活性受到SA抑制,且随外源SA的浓度增加而下降,导致超氧自由基上升。SA也能抑制CAT和APX活性,CAT活性随SA浓度增加而降低,APX活性也有这种趋势。尽管SA提高了POD活性,但由于CAT和APX活性受到抑制,H2 O2 含量上升。GR活性也受到SA抑制,呈现随SA浓度增加而降低的趋势。经5mmol·L-1SA处理的叶片内MDA含量下降,平均比对照低35.19% ,表明低浓度SA能降低冰冻引起的伤害。

关键词: 冰冻胁迫, 水杨酸, 抗氧化酶, 水曲柳

Abstract:

The effects of exogenous salicylic acid(SA) (5 and 10 mmol·L-1)on activities of antioxidant enzymes in seedlings of Fraxinus mandshurica were analyzed in the present paper.Activities of superoxide dismutase(SOD) were decreased by SA,and reduced with the increase of SA concentrations,resulting in the increase of superoxide radicals.The activities of catalase(CAT) and ascorbate peroxidase(APX) were inhibited by SA,and their activities reduced with the increase of SA concentrations.Although the activities of peroxidase(POD)increased,H2O2 levels increased in leaves with SA treatment because of the inhibition of CAT and APX.The activities of glutathione reductase(GR) was decreased by SA,and reduced with the increase of SA concentration. In general,malondialdehyde(MDA) levels in leaves with 5 mmol·L-1SA treatment were 35.19% lower than that of control,suggesting low concentration of SA reduced freezing injury in seedlings of Fraxinus mandshurica.

Key words: Freezing stress, Salicylic acid, Antioxidant enzymes, Fraxinus mandshurica