松属线粒体基因序列变异研究

摘要/Abstract

摘要：

本研究利用PCR和PCR产物直接序列分析技术,获得了分属于松属(Pinus)两个亚属Pinus和Strobus12个松树种coxI、atp6和orf25三个线粒体基因DN A片段的序列。发现coxI基因的DNA序列在属内没有任何变异,但atp6和orf25在两个亚属间和亚属内出现了较多的碱基置换。这说明尽管裸子植物线粒体基因有较多的RNA编辑(RNA editing)存在,可促进DNA序列的变异,但促进程度因不同基因而异。依据atp6和orf25所做的系统进化树不仅与分类结果一致,而且进一步表明了亚属内种间的亲缘关系。尽管线粒体基因序列变异比叶绿体和核DNA变异小,线粒体基因编码序列变异仍适于研究裸子植物亚属甚至组间的系统进化关系。

关键词: 松树, 线粒体基因, 系统进化分析, 序列变异

Abstract:

Mitochondrial (mt) coxI, atp6 and orf25 DNA fragments for 12 species, which belong to two subgenera Pinus and Strobus of the genus Pinus, were amplified by polymerase chain reaction using gene specific primers. Their partial sequences were obtained by directly sequencing the PCR products with the same sets of primers. No sequence difference in coxI was observed among 12 species. However, nucleotide substitutions in both atp6 and orf25 between and within two subgenera were found. These results indicated that the variation of mitochondrial gene sequences were gene dependent, although the intensive RNA editing existed in mitochondrial genome could stimulate the divergence of the genomic DNA sequences. The phylogenetic trees of 12 species using atp6 and orf25 sequences agreed with their taxonomy positions, and the genetic relationships between the species within each subgenus were resolved additionally. This suggests that the mitochondrial gene sequences can be used in the phylogenetic analysis below the genus level in gymnosperms.

Key words: Pinus, Mitochondrial gene, Phylogenetic analysis, Sequence divergence

卢孟柱,AlfredE.Szmidt. 松属线粒体基因序列变异研究[J]. 林业科学, 1999, 35(4): 14-20.

Mengzhu Lu,E.Szmidt Alfred. THE SEQUENCE VARIATION OF MITOCHONDRIAL GENES IN PINUS[J]. Scientia Silvae Sinicae, 1999, 35(4): 14-20.

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参考文献 0

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