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林业科学 ›› 2009, Vol. 12 ›› Issue (11): 32-35.doi: 10.11707/j.1001-7488.20091106

• 论文 • 上一篇    下一篇

部分箬竹属植物的荧光AFLP分析*

牟少华1 彭镇华2 孙启祥2 高志民1 李雪平1   

  1. 1.国际竹藤网络中心 国家林业局竹藤科学与技术重点开放实验室 北京 100102; 2.中国林业科学研究院林业研究所 北京 100091
  • 收稿日期:2008-08-28 修回日期:1900-01-01 出版日期:2009-11-25 发布日期:2009-11-25
  • 通讯作者: 彭镇华
  • 基金资助:
     

Genetic Diversity of Indocalamus Determined by AFLP Marker

Mu Shaohua1, Peng Zhenhua2, Sun Qixiang2, Gao Zhimin2, Li Xueping1   

  1. 1. Key Laboratory of Bamboo and Rattan Science and Technology of State Forestry Administration International Center for Bamboo and Rattan Beijing 100102;2. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091
  • Received:2008-08-28 Revised:1900-01-01 Online:2009-11-25 Published:2009-11-25
  • Supported by:
     

摘要:

利用AFLP技术,选择EcoRⅠ/MseⅠ这一酶切组合,应用9对(EcoRⅠ+3)/(MseⅠ+3)引物组合进行选择性扩增,检测16种箬竹和5个形态相似的外源竹种的基因组DNA 多态性。在21个样品基因组中共获得1 367条清晰的谱带,其中共有条带115条,特异性带273条,特异性缺失条带72条。在16个箬竹样品基因组中共获得1 193条清晰的谱带,其中共有条带190条,特异性带177条,特异性缺失条带98条。这些特异性带或特异性缺失条带可作为识别不同竹种的分子标记。聚类结果表明: 所有的箬竹属植物聚成一类,其他5种外源植物聚成一类。此外,AFLP分析提供的分子证据,支持耿氏分类系统(1996),将天目箬竹归入阔叶箬竹中。

关键词: 箬竹属, AFLP, 多样性

Abstract:

Amplified fragment length polymorphism (AFLP) was used to study DNA diversity of 16 species in Indocalamus and other 5 similar bamboo species. Genomic DNA was digested with EcoRⅠ and MseⅠ enzymes and amplified with 9 (EcoRⅠ+3)/(MseⅠ+3) primer combinations. In the 21 samples, 9 EcoRⅠ-MseⅠ AFLP primer combinations produced 1 367 legible bands, of which there were 115 common bands, 273 specific bands and 72 specific missing bands. In the 16 samples of Indocalamus, 1 193 legible bands were obtained by using 9 primer combinations,with 190 common bands, 177 specific bands and 98 specific missing bands. All the specific bands and specific missing bands would be used as molecular markers to identify these species. The AFLP markers indicated that I. Migoi and I. Latifolius were the same species.

 

Key words: Indocalamus, AFLP, diversity

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