4种植物生长调节剂对红花玉兰嫩枝扦插生根的影响

doi:10.11707/j.1001-7488.20190704

摘要/Abstract

摘要： [目的]应用不同种类不同浓度的生长调节剂对红花玉兰嫩枝进行处理，以提高红花玉兰嫩枝扦插生根率，为红花玉兰的快速繁殖、栽培及推广提供理论支持和技术指导。[方法]以5年生红花玉兰实生苗为采穗母树，选择其当年生半木质化枝条制备插穗作为试验材料，采用双因素完全随机区组设计，研究GGR₆、NAA、IBA、IAA 4种植物生长调节剂以及200、500、800、1000、2000、3000 mg·L^-1 6种不同浓度对红花玉兰嫩枝扦插生根的影响。扦插80天后，调查统计其生根率、生根数量、平均根长、平均根直径和生根性状评分率，并运用主成分分析法对各处理组合的生根效果进行综合评价。[结果]NAA处理生根率以及根系形态表现均处于较高水平，其中1000 mg·L^-1的溶液处理扦插根系综合质量最好，平均生根数量为13.3，相比清水对照组增加了12根；平均根长为14.33 cm，为清水对照组的2.7倍；平均根直径为2.44 mm，比清水对照组增粗0.60 mm；生根性状评分率为55.4%，相比清水对照组提高22.1%；生根率最高，达到53.3%，与其他浓度存在显著差异，约为清水对照组的16倍。[结论]4种植物生长调节剂中NAA处理插穗效果最好，而在其6种不同浓度中，1000 mg·L^-1的处理生根效果最好。

关键词: 红花玉兰, 扦插, 植物生长调节物质, 生根率, 根系形态指标

Abstract: [Objective] The aim of this study was to improve the rooting rate of cutting of M. wufengensis, and provide theoretical support and guidance for the rapid propagation, cultivation and promotion of M. wufengensis.[Method] The semi-lignified branches from 5-year-old Magnolia wufengensis seedlings were collected as the propagation materials. A completely randomized block design with two factors, including four types of plant growth regulators of GGR₆,NAA,IBA,and IAA, and six concentrations of 200,500,800,1 000,2 000,3 000 mg·L^-1,were used. The effects of different types and concentrations of plant growth regulars on rooting parameters of M. wufengensis, i.e. rooting rate, number of adventitious roots, average root length, average root diameter, and root effective traits rate, were investigated. And then the principal component analysis was used to comprehensively evaluate the effects of each treatment combination on rooting.[Result] The results showed that the rooting rate and root morphology of NAA solution treatment was all at a high level among the four plant growth regulators. When the cuttings was soaked in 1 000 mg·L^-1 NAA solution, the cutting root had the best quality. The average number of adventitious roots was 13.3, which was 12 more than that of the control group treated with water. The average root length was 14.33 cm, which was about 2.7 times of the control group. The average root diameter was 2.44 mm,which was 0.60 mm thicker than that of the control group. The root effective traits rate was 55.4%, which was 22.1% higher than that of the control group. The rooting rate of NAA 1 000 mg·L^-1 treatment was the highest, reaching 53.3%, significantly different from other concentrations, and 16 times higher than that of the control group.[Conclusion] The NAA solution had the better rooting effects than the other three types of growth regulator, and the best concentration of NAA was 1 000 mg·L^-1. The research has greatly increased the rooting rate of M. wufengensis, and has provided theoretical basis and technical guidance for the large-scale propagation of germplasm resources of M. wufengensis.

Key words: Magnolia wufengensis, cuttage propagation, plant growth regulators, rooting rate, root morphology parameter

中图分类号:

S718.43

王艺, 贾忠奎, 马履一, 邓世鑫, 朱仲龙, 桑子阳. 4种植物生长调节剂对红花玉兰嫩枝扦插生根的影响[J]. 林业科学, 2019, 55(7): 35-45.

Wang Yi, Jia Zhongkui, Ma Lüyi, Deng Shixin, Zhu Zhonglong, Sang Ziyang. Effects of Four Plant Growth Regulators on Rooting of the Softwood Cutting of Magnolia wufengensis[J]. Scientia Silvae Sinicae, 2019, 55(7): 35-45.

参考文献

安三平,王丽芳,石红,等.2011.蓝云杉不同品种扦插生根能力和生根特性研究.林业科学研究, 24(4):512-516.
(An S P, Wang L F, Shi H, et al. 2011. Rooting ability and properties of different varieties of Piceapungenscutting. Forest Research, 24(4):512-516.[in Chinese])
陈庆生,周鹏,张敏,等. 2016.激素和插穗处理对乌饭树扦插生根的影响.东北林业大学学报, 44(4):41-47.
(Chen Q S, Zhou P, Zhang M, et al. 2016. Influence of different treatment of cuttings and hormone on rooting of cutting of Vaccinum bracteatum. Journal of Northeast Forestry University, 44(4):41-47.[in Chinese])
龚弘娟,李洁维,蒋桥生,等. 2008.不同植物生长调节剂对中华猕猴桃扦插生根的影响.广西植物, 28(3):359-362.
(Gong H J, Li J W, Jiang Q S, et al. 2008. Effects of different plant regulators on rooting of Actinidia chinensis cutting. Guihaia, 28(3):359-362.[in Chinese])
胡勐鸿,欧阳芳群,贾子睿,等. 2014.欧洲云杉扦插生根影响因子研究于生根力优良单株选择.林业科学, 50(2):42-49.
(Hu M H, Ouyang Q F, Jia Z R, et al. 2014. Factors affecting rooting of Picea abiesshoot cuttings and individual selection with high rooting ability. Scientia Silvae Sinicae, 50(2):42-49.[in Chinese])
李玲莉,田中,桑子阳. 2013.红花玉兰一年生播种苗的生长规律研究.北方园艺, (24):49-52.
(Li L L, Tian Z, Sang Z Y. 2013. Study on growth regularity of annual seedlings of Magnolia wufengensis. Northern Horticulture, (24):49-52.[in Chinese]
李修鹏,俞慈英,董韩忠,等. 2002.木兰科植物无性繁殖研究.林业科技开发, 16(3):40-42.
(Li X P, Yu C Y, Dong H Z, et al. 2002. Study on asexual propagation of Magnoliaceae. China Forestry Science and Technology Development, 16(3):40-42.[in Chinese])
廖伟彪,张美玲,杨永花,等. 2012.植物生长调节剂浓度和处理时间对月季扦插生根的影响.甘肃农业大学学报, 47(3):47-51.
(Liao W B, Zhang M L, Yang Y H, et al. 2012. Effects of different plant growth regulator concentrations and processing times on root of Rosa chinensis cuttings. Journal of Gansu Agricultural University, 47(3):47-51.[in Chinese])
梁玉婷,邓永成,夏文胜,等. 2018.吲哚乙酸和萘乙酸对红花玉兰嫩枝扦插生根的影响.安徽农学通报, 24(5):87-88.
(Liang Y T, Deng Y C, Xia W S, et al. 2018. Study of indole-3-acetic acid and 1-Naphthaleneacetic acid treatment on rooting of softwood-cutting of Magnolia wufengensis L. Y. Ma et L. R. Wang. Anhui Agricultural Science Bulletin, 24(5):87-88.[in Chinese])
林艳,詹亚光,刘玉喜,等. 1996.白桦嫩枝扦插不定根形成的解剖观察.东北林业大学学报, 24(3):15-18.
(Lin Y, Zhan Y G, Liu Y X, et al. 1996. The anatomical study on formation of adventitious root of softwood cutting in Betula Platyphylia. Journal of Northeast Forestry University, 24(3):15-18.[in Chinese])
刘桂丰,杨传平,曲冠正,等. 2001.落叶松杂种插穗生根过程中4种内源激素的动态变化.东北林业大学学报, 29(6):1-3.
(Liu G P, Yang C P, Qu G Z, et al. 2001. Dynamic changes of four endogenous hormones during the rooting of Larch Hybrids. Journal of Northeast Forestry University, 29(6):1-3.[in Chinese])
刘勇,肖德兴,黄长干,等. 1997.板栗嫩枝扦插生根解剖学特征研究.园艺学报, 24(1):8-12.
(Liu Y, Xiao D X, Huang C G, et al. 1997. The anatomical feasturestudy on the rooting of the soft wood cuttings of Castanea mollissima Blume. Acta Horticulturae Sinica, 24(1):8-12.[in Chinese])
刘正祥,张华新,刘涛. 2007.省沽油硬枝扦插生根特性.东北林业大学学报, 35(7):13-15.
(Liu Z X, Zhang H X, Liu T. 2007. Rooting characteristics of hard-wood cuttings of Staphylea bumalda. Journal of Northeast Forestry University, 35(7):13-15.[in Chinese])
马履一,王罗荣,贺随超,等. 2006.中国木兰科木兰属一新种.植物研究, 26(1):4-7.
(Ma L Y, Wang L R, He S C, et al. 2006. A new species of Magnoliaceae from China. Plant research, 26(1):4-7.[in Chinese])
任俐,刘小东,李耀文. 2006.三种植物激素对紫丁香扦插的影响.哈尔滨商业大学学报:自然科学版, 22(2):33-36.
(Ren L, Liu X D, Li Y W. 2006. Effects of three plant hormones on cuttings of Syringa oblata Lindl. Journal of Harbin University of Commerce:Natural Science Edition, 22(2):33-36.[in Chinese])
沈作奎. 2008.紫玉兰嫩枝扦插繁殖研究.安徽农业科学, 36(33):14505-14508.
(Shen Z K. 2008. Study on cutting propagation of Syringa oblata Lindl. Anhui Agricultural Sciences, 36(33):14505-14508.[in Chinese])
舒常庆,赵西梅,杨臻,等. 2007.3种女贞属植物的扦插繁殖研究.华中农业大学学报, 26(3):390-393.
(Shu C Q, Zhao X M, Yang Z, et al. 2007. Study on cutting propagation of three species of Ligustrum. Journal of Huazhong Agricultural University, 26(3):390-393.[in Chinese])
王慧,楼炉焕,朱小楼. 2010.不同植物生长调节物质对西南卫矛和卫矛扦插生根的影响.浙江林学院学报, 27(1):155-158.
(Wang H, Lou L H, Zhu X L. 2010. Effects of different plant growth regulators on the rooting of cuttings of Euonymus argus and Euonymus. Journal of Zhejiang Forestry College, 27(1):155-158.[in Chinese])
王晓玲,马履一,贾忠奎,等. 2011.红花玉兰研究进展.北方园艺, (16):202-205.
(Wang X L, Ma L Y, Jia Z K, et al. 2011. Research progress of Magnolia wufengensis. Northern Horticulture, (16):202-205.[in Chinese])
王亚玲. 2004.木兰科植物的无性繁殖.中国野生植物资源, 23(3):56-58.
(Wang Y L. 2004. The asexual reproduction of Magnoliaceae plant. Chinese Wild Plant Resources, 23(3):56-58.[in Chinese])
王军辉,张建国,张守攻,等. 2006.青海云杉硬枝扦插的激素、年龄和位置效应研究.西北农林科技大学学报:自然科学版, 34(7):65-71.
(Wang J H, Zhang J G, Zhang S G, et al. 2006. Research of hormone,age and position effect ofhardwood cutting in Picea crassifolia Kom. Journal of Northwest Sci-Tech University of Agriculture and Forestry:Natural Science Edition, 34(7):65-71.[in Chinese])
徐明艳,刘志恩,邓桂香,等. 2015.不同外源激素对华山松扦插生根的影响.广东林业科技, 31(5):56-59.
(Xu M Y, Liu Z E, Deng G X, et al. 2015. Influence of different exogenous hormone treatments on Pinus armandii cutting rooting. Guangdong Forestry Science and Technology, 31(5):56-59.[in Chinese])
占玉芳,滕玉丰,甄伟玲. 2008.全光照喷雾四翅滨藜嫩枝扦插试验.东北林业大学学报, 36(7):10-11.
(Zhan Y F, Teng Y F, Zhen W L. 2008. Tender branch cutting of Atyipiex canexcens under full sunlight and water-spaying. Journal of Northeast Forestry University, 36(7):10-11.[in Chinese])
张晓平,方炎明. 2003.杂种鹅掌楸插穗不定根发生与发育的解剖学观察.植物资源与环境学报, 12(1):10-15.
(Zhang X P, Fang Y M. 2003. Anatomical observation of the origin and development of adventitious roots in Hybrid Tuliptrees during cutting. Journal of Plant Resources and Environment, 12(1):10-15.[in Chinese])
张应团. 2000.紫玉兰绿枝扦插生根率与采条时期的关系.江苏林业科技, 27(2):16-19.
(Zhang Y T. 2000. A study on the relationship between Magnolia lilifora Desr. green branch cuttage rooting rate and cutting time. Jiangsu Forestry Science and Technology, 27(2):16-19.[in Chinese])
张玉臣,周再知,梁坤南,等. 2010.不同植物生长调节剂对白木香扦插生根的影响.林业科学研究, 23(2):278-282.
(Zhang Y C, Zhou Z Z, Liang K N, et al. 2010. Effect of different plant growth regulators on the rooting of Aquilaria sinensis cuttings. Foret Research, 23(2):278-282.[in Chinese])
郑均宝. 1989.树木的营养繁殖.北京:中国林业出版社.
(Zheng J B. 1989. Vegetative reproduction of trees. Beijing:Chinese Forestry.[in Chinese])
宗树斌,鲍荣静,段春玲. 2008.宝华玉兰扦插繁殖技术研究.山东林业科技, (6):39-41.
(Zong S B, Bao R J, Duan C L. 2008. Study on cuttagepropagation technique of Magnolia zenii. Shandong Forestry Science and Technology, (6):39-41.[in Chinese])
Bouza L, Jacques M, Sotta B, et al. 1994. Relation between auxin and cytokine in contents and in vitro rooting of tree peony (Paeonia suffruticosa Andr.). Plant Growth Regulation, 15(1):69-73.
Ermel F F, Vizoso S, Charpentier J P, et al. 2000. Mechanisms of primordium formation during adventitious root development from walnutcotyledon explants. Planta, 211(4):563-574.
Haecker A, Gross-Hardt R, Geiges B, et al. 2004. Expression dynamics of WOX genes mark cell fate decisions during earlyembryonic patterning in Arabidopsis thaliana. Development, 131(3):657-668.
Haissig B E. 1972. Meristematic activity during adventitious root primordium development influences of endogenous auxin and applied gibberellic acid. Plant physiology, 49 (6):886-892.
Henrique A, Carnpinhos E N, One E O, et al. 2006. Effect of plant growth regulators in the rooting of Pinus cuttings. Brazilian Archives of Biology and Technology, 49(2):189-196.
Legué V, Rigal A, Bhalerao R P. 2014. Adventitious root formation in tree species:involvement of transcription factors. Physiololgia Plantarum, 151(2):192-198.
Liao W B, Xiao H L, Zhang M L. 2010. Effect of nitric oxide and hydrogen peroxide on adventitious root development from cuttings of ground-cover chrysanthemum and associated biochemical changes. Journal of Plant Growth Regulation, 29(3):338-348.
Liu B B, Wang L, Zhang J, et al. 2014. WUSCHEL-related homeobox genes in Populus tomentosa: diversified expression patterns and afunctional similarity in adventitious root formation. BMC Genomics, (15):296-310.
Quan J N, Zhang C X, Zhang S, et al. 2014a. Molecular cloning and expression analysis of the MTN gene during adventitious root development in IBA-induced tetraploid black locus. Gene, 553(2):140-150.
Quan J N, Zhang S, Zhang C X, et al. 2014b. Molecular cloning, characterization and expressionanalysts of the SAMS gene during adventitious root development in IBA-induced tetraploid black locust. PLoS One, 9(10):709-721.
Rigal A, Yordanov Y S, Perrone I, et al. 2012. The AINTEGUMENTA LIKE1 homeotic transcription factor PtAIL1 controls the formation of adventitious rootprimordia in poplar. Plant Physiology, 160(4):1996-2006.
Sanchez C, Vielba J M, Ferro E, et al. 2007. Two SCARE-CROW-LIKE genes are induced in response to exogenousauxin in rooting-competent cuttings of distantly related forest species. Tree Physiology, 27(10):1459-1470.
Smith D L, Fedoroff N V. 1995. LRP1, a gene expressed inlateral and adventitious root primordial of Arabidopsis. The Plant Cell, 7(6):735-745.
Smolka A, Welander M, Olsson P, et al. 2009. Involvement of the ARRO-1 gene in adventitious rootformation in apple. Plant Science, 177(6):710-715.
Trupiano D, Yordanov Y, Regan S, et al. 2013. Identification, characterization of an AP2/ERF transcription factor that promotes adventitious, lateral root formation in Populus. Planta, 238(2):271-282.
Vielba J M, Diaz-Sala C, Ferro E, et al. 2011. CsSCL1 isdifferentially regulated upon maturation in chestnut microshoots and is specifically expressed in rooting-competentcells. Tree Physiology, 31(10):1152-1160.
Xing H Y, Pudake R N, Guo G G. 2011. Genome-wide identification and expression profiling of auxin response factor (ARF)gene family in maize. BMC Genomics, (12):178-190.

[1]	杜常健, 孙佳成, 陈炜, 纪敬, 江泽平, 史胜青. 侧柏古树实生树和嫁接树的扦插生理和解剖特性比较[J]. 林业科学, 2019, 55(9): 41-49.
[2]	秦爱丽, 简尊吉, 马凡强, 郭泉水, 郑祥坤. 母树年龄、生长调节剂、容器与基质对崖柏嫩枝扦插的影响[J]. 林业科学, 2018, 54(7): 40-50.
[3]	文书生, 何绒绒, 郑佳康, 田如男. 牡丹组织培养技术研究进展[J]. 林业科学, 2018, 54(10): 143-155.
[4]	王书胜, 单文, 张乐华, 杜有新, 李晓花, 王凯红. 基质和IBA浓度对云锦杜鹃扦插生根的影响[J]. 林业科学, 2015, 51(9): 165-172.
[5]	欧阳芳群, 付国赞, 王军辉, 马建伟, 安三平, 王美琴, 李悦. 欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J]. 林业科学, 2015, 51(3): 155-162.
[6]	张乐华, 王书胜, 单文, 李晓花, 王凯红, 王兆宏. 基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J]. 林业科学, 2014, 50(3): 45-54.
[7]	胡勐鸿, 欧阳芳群, 贾子瑞, 王军辉, 张宋智, 马建伟. 欧洲云杉扦插生根影响因子研究与生根力优良单株选择[J]. 林业科学, 2014, 50(2): 42-49.
[8]	魏海蓉, 陈新, 宗晓娟, 王甲威, 张庆霞, 张道辉, 刘庆忠. 甜樱桃矮化砧‘吉塞拉6号’扦插过程中氧化酶活性和碳氮含量变化[J]. 林业科学, 2013, 49(9): 172-177.
[9]	赵云龙, 陈训, 李朝婵. 糙叶杜鹃扦插生根过程中生理生化分析[J]. 林业科学, 2013, 49(6): 45-51.
[10]	杨尚尚, 苑兆和, 李云, 李芹, 尹燕雷, 冯立娟, 招雪晴. 石榴‘泰山红’的花粉萌发生物学特性[J]. 林业科学, 2013, 49(10): 48-53.
[11]	孙红梅;安晶;王春夏;谢佳;陶承光. 激素和变温处理对百合鳞片扦插繁殖的影响[J]. 林业科学, 2011, 47(3): 39-45.
[12]	张猛;王丹;汤浩茹;任少雄;刘仁道. 费约果茎解剖结构与插条不定根的形成[J]. 林业科学, 2010, 46(7): 183-187.
[13]	冯健<sup></sup>齐力旺<sup></sup>孙晓梅<sup></sup>张守攻<sup></sup>. 落叶松扦插生根过程SSH文库构建及部分基因的表达分析[J]. 林业科学, 2010, 46(6): 27-34.
[14]	王新建. 何威张秋娟王念祝亚军朱延林. 豫楸1号扦插生根过程中营养物质含量及氧化酶类活性的变化*[J]. 林业科学, 2009, 12(4): 156-161.
[15]	扈红军曹帮华尹伟伦翟明普. 榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术*[J]. 林业科学, 2008, 44(6): 66-72.