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林业科学 ›› 2019, Vol. 55 ›› Issue (6): 160-166.doi: 10.11707/j.1001-7488.20190619

• 研究简报 • 上一篇    下一篇

云杉幼树根系分泌物对2种草本植物种子萌发和幼苗生长的影响

蒋铮1,3, 于倩楠1,4, 乔明锋1,2, 肖娟5, 张子良1,3, 尹华军1,5   

  1. 1. 中国科学院山地生态恢复与生物资源利用重点实验室 生态恢复与生物多样性保育四川省重点实验室 中国科学院成都生物研究所 成都 610041;
    2. 四川旅游学院 成都 610100;
    3. 中国科学院大学 北京 100049;
    4. 西南交通大学地球科学与环境工程学院 成都 611756;
    5. 西华师范大学环境科学与工程学院 南充 637000
  • 收稿日期:2018-02-08 修回日期:2019-02-21 出版日期:2019-06-25 发布日期:2019-07-11
  • 基金资助:
    四川省前沿重点研发项目(2017SZ0038);四川省青年科技基金项目(2016JQ0037);国家自然科学基金项目(31700387);中国科学院山地生态恢复与生物资源利用重点实验室开放基金(KXYSWS1601)。

Effects of Root Exudates from Picea asperata Seedlings on the Seed Germination and Seedling Growth of Two Herb Species

Jiang Zheng1,3, Yu Qiannan1,4, Qiao Mingfeng1,2, Xiao Juan5, Zhang Ziliang1,3, Yin Huajun1,5   

  1. 1. CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of Biology, Chinese Academy of Sciences Chengdu 610041;
    2. Sichuan Tourism University Chengdu 610100;
    3. University of Chinese Academy of Sciences Beijing 100049;
    4. Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University Chengdu 611756;
    5. College of Environmental Science and Engineering, China West Normal University Nanchong 637000
  • Received:2018-02-08 Revised:2019-02-21 Online:2019-06-25 Published:2019-07-11

摘要: [目的]探究西南亚高山人工针叶林主要造林树种粗枝云杉根系分泌物输入对林下草本植物种子萌发和幼苗生长的影响,揭示根系分泌物对林下植被更新和群落多样性的潜在生态效应。[方法]通过前期在野外原位收集根系分泌物,后期在实验室人工添加的方式,从种子萌发、幼苗生物量、幼苗形态3个方面来探究根系分泌物对西南亚高山人工云杉林生境内的2种草本植物白车轴草和紫花苜蓿的影响。[结果]1)在种子萌发方面,根系分泌物添加降低了2种草本植物的种子发芽率、发芽势和发芽指数,且紫花苜蓿种子在处理间差异显著。2)在幼苗生物量方面,2种草本植物幼苗鲜质量、干质量在根系分泌物处理下均呈下降趋势,而且紫花苜蓿幼苗处理间差异显著。3)在幼苗形态特征方面,根系分泌物添加降低了2种草本植物的总长度、总表面积、总体积,且幼苗总长度处理间存在显著差异。4)根系分泌物处理下,2种草本植物的各项化感效应指数(RI值)均小于0,而且根系分泌物处理造成的2种植物化感效应综合指数(SE值)小于0,表明根系分泌物添加对2种草本植物种子萌发和幼苗生长均具有抑制作用,且对紫花苜蓿种子的抑制作用更强。5)UPLC-MS分析表明,云杉幼树根系分泌物中检测出多种酚酸类物质。[结论]云杉幼树根系分泌物输入对林下草本植物种子萌发及幼苗生长均产生了抑制效应。根系分泌物中的酚酸类物质可能是导致根系分泌物处理下草本植物种子萌发和幼苗生长受到抑制的内在驱动机制。

关键词: 根系分泌物, 种子萌发, 幼苗生长, 亚高山人工针叶林

Abstract: [Objective] This study aimed to investigate the ecological effects of root exudates input on herb species from seed germination and seedling growth.[Method] We performed an experiment on acceptors(Trifolium repens and Medicago sativa) in which in situ exudates were collected from Picea asperata seedlings.[Result] 1) Root exudates decreased the germination rate, germination vigor and germination index of the two herb species seeds, and significant differences were observed on the M. sativa seedlings. 2) Similarly, both the fresh weight and dry weight of two herb species seedlings were decreased under root exudates treatments, and significant differences were also observed on M. sativa seedlings. 3) Root exudates additions reduced the total length, total surface area and total volume of two herb species seedlings. Among them, significant differences were found only for the total length. 4) Synthesis effect was a vital index of allelopathy. Further analysis showed that the synthesis effects of root exudates on seed germination and seedling growth of two herb species seedlings were negative. And the allelopathy to M. sativa was stronger than that to T. repens. 5) The analysis of root exudates showed that numerous phenolic acids were found in the root exudates of P. asperata seedlings.[Conclusion] 1) Our result manifested that the root exudates from P. asperata seedlings had obvious inhibitory effects on the seed germination and seedling growth of the herb species. 2) Numerous phenolic acids in the root exudates maybe one of major reasons for the observed inhibiting effects of root exudates.

Key words: root exudates, seed germination, seedling growth, subalpine coniferous plantation

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