偏肿革裥菌Lg-mnp2基因在构巢曲霉中的表达

摘要/Abstract

Abstract:

The aim of this study was to enhance manganese peroxidase (MnP) gene express by Lenzites gibbosa MnP production for using Aspergillus nidulans. In this study, an auxotrophic strain, TN02A7, of A.nidulans was used as the host of L. gibbosa.The conidiophores of TN02A7 were used to produce the protoplasts by different cell wall lyases, including lywallzyme, cellulase and snailase which were mixed together by 1:1:1 for effectively break down the wall and produce protoplasts. PEG/CaCl₂ were used to mediate transformation of L. gibbosa, containing a gene encoding for manganese peroxidase 2 (Lg-MnP2), into the TN02A7 protoplast. The newly obtained transformant strain, TN02A7-Lg-mnp2 , and TN02A7 were cultured in the same shaking medium containing lignin and the MnP activity was detected. The result showed that TN02A7-Lg-mnp2 could produce MnP activity up to 17 U ·L^-1 in 96 h, while TN02A7 did not produce MnP activity at any time, indicating that the gene Lg-mnp2 had been successfully transformed into TN02A7-Lg-mnp2 and expressed in lignin environment. This study provided a new method to produce MnP.

Key words: Lenzites gibbosa, Lg-mnp2, Aspergillus nidulans, transformation, expression

中图分类号:

S718.81

胡美美, 池玉杰. 偏肿革裥菌Lg-mnp2基因在构巢曲霉中的表达[J]. 林业科学, 2014, 50(2): 139-143.

Hu Meimei, Chi Yujie. Expression of the Gene Lg-mnp2 Encoding for Manganese Peroxidase 2 (MnP2) from White-Rot Fungus Lenzites gibbosa in Aspergillus nidulans[J]. Scientia Silvae Sinicae, 2014, 50(2): 139-143.

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