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林业科学 ›› 2014, Vol. 50 ›› Issue (11): 52-57.doi: 10.11707/j.1001-7488.20141107

• 论文与研究报告 • 上一篇    下一篇

麻竹转录因子DlSCL6的基因克隆及在拟南芥中异位表达

杨丽1, 陈东亮1,2, 彭镇华, 赵韩生1, 高志民1   

  1. 1. 国际竹藤中心 竹藤科学与技术重点开放实验室 北京 100102;
    2. 北京市农林科学院 北京农业生物技术研究中心 北京 100097;
    3. 中国林业科学研究院林业研究所 北京 100091
  • 收稿日期:2014-01-06 修回日期:2014-04-18 出版日期:2014-11-25 发布日期:2014-12-04
  • 通讯作者: 高志民
  • 基金资助:

    国家林业局948项目(2011-4-55).

Cloning of Transcription Factor DlSCL6 from Dendrocalamus latiflorus and Its Ectopic Expression in Arabidopsis thaliana

Yang Li1, Chen Dongliang1,2, Peng Zhenhua, Zhao Hansheng1, Gao Zhimin1   

  1. 1. Key Laboratory on the Science and Technology of Bamboo and Rattan International Center for Bamboo and Rattan Beijing 100102;
    2. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences Beijing 100097;
    3. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091
  • Received:2014-01-06 Revised:2014-04-18 Online:2014-11-25 Published:2014-12-04

摘要:

GRAS家族HAM(Hairy Meristem)亚家族是一类转录因子,对植物的生长发育和形态建成具有重要作用.利用RT-PCR和RACE技术从麻竹中得到一个HAM同源基因,命名为DlSCL 6.该基因全长2 006 bp,含有5'非编码区129 bp,3'非编码区266 bp,编码区1 611 bp.DlSCL6蛋白具有LHRⅠ,VHIID,LHRⅡ,PFYRE,SAM 5个保守域,且与某些单子叶植物的SCL6蛋白有较高的一致性,与拟南芥有部分一致性,为43%.DlSCL 6基因在大肠杆菌中表达,获得分子量约为60 kDa的重组蛋白.在拟南芥中正义表达DlSCL 6基因的植株营养期延长,开花延迟,植株粗壮,莲座叶数量增加; 而转反义基因的植株开花提前,植株瘦弱,且莲座叶数量明显减少.由此表明,DlSCL 6基因能影响转基因植株茎端分生组织的分生状态,从而导致形态建成的变化.

关键词: 麻竹, 转录因子, 拟南芥, 异位表达

Abstract:

HAM (Hairy Meristem) subfamily in GRAS family is a group of transcription factors that play important roles in plant growth and morphogenesis. By using RT-PCR and RACE technique, a HAM homologous gene, DlSCL 6 was cloned from Dendrocalamus latiflorus. This gene has 2 006 bp in full-length including the 5' untranslated region (UTR) of 129 bp, 3' UTR of 266 bp, and coding region of 1 611 bp. DlSCL6 protein has five conserved domains (LHR Ⅰ, VHIID, LHR Ⅱ, PFYRE, and SAM), and is relatively high consistent to SCL6 proteins in some monocots, and some consistent of 43% to the Arabidopsis thaliana. A recombinant protein with approximately 60 kDa was obtained in the expression of this gene in Escherichia coli. The sense transgenic plants derived from ectopic expression of DlSCL 6 in A. thaliana showed prolonged vegetative growth, delayed flowering, and increased rosette leaf number, while the antisense transgenic plants had early flowering and thin plants and significantly reduced rosette leaves number. This study indicates that DlSCL 6 gene can influence the shoot apical meristem status, and hence result in changes in morphogenesis.

Key words: Dendrocalamus latiflorus, transcription factor, Arabidopsis thaliana, ectopic expression

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