外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响

doi:10.11707/j.1001-7488.20130609

林业科学 ›› 2013, Vol. 49 ›› Issue (6): 60-67.doi: 10.11707/j.1001-7488.20130609

外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响

杨玲, 王佳男, 边磊, 沈海龙

东北林业大学林学院林木遗传育种国家重点实验室哈尔滨 150040

收稿日期:2012-08-14 修回日期:2013-01-23 出版日期:2013-06-25 发布日期:2013-07-16
通讯作者: 沈海龙
基金资助:
黑龙江省教育厅科学技术指导项目(11553034); 中央高校基本科研业务费专项资金(DL10BA04)。

Effects of Exogenous Nitric Oxide on Embryo Germination and ROS Accumulation in Seedling Growth Initial Stage of Sorbus pohuashanensis

Yang Ling, Wang Jianan, Bian Lei, Shen Hailong

School of Forestry, Northeast Forestry University State Key Laboratory of Tree Genetics and Breeding Harbin 150040

Received:2012-08-14 Revised:2013-01-23 Online:2013-06-25 Published:2013-07-16

摘要/Abstract

摘要：

以花楸树成熟合子胚胎为材料,利用室内培养皿发芽法研究外源NO供体(SNP)、NO合成抑制剂(PTIO)、脱落酸(ABA)以及乙烯受体抑制剂(NBD)对花楸树胚胎萌发和幼苗发育初期活性氧(ROS)积累的影响。结果表明: 光照条件下SNP预处理可减弱PTIO或ABA对胚胎萌发的抑制作用,但不能减弱NBD处理对胚胎萌发的抑制作用。SNP预处理显著增加幼苗叶片中叶绿素含量,但这种增加效应不能被PTIO解除。PTIO抑制SNP预处理引起的幼苗叶片中过氧化氢(H₂O₂)含量和超氧阴离子含量的增加。SNP预处理解除PTIO对根部超氧阴离子含量的抑制作用。研究结果为阐明胚胎萌发过程中反应氮调节的(NO依赖型)休眠解除和ROS 积累的关系奠定基础。

关键词: 花楸树, 胚胎萌发, 一氧化氮, 脱落酸, 乙烯

Abstract:

In this paper, mature zygotic embryo of Sorbus pohuashanensis were used to study effects of an exogenous nitric oxide donor (sodium nitroprusside, SNP), NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), abscisic acid (ABA) and 2,5-norbornadiene (NBD)—an inhibitor of the ethylene receptor on the embryo germination and reactive oxygen species (ROS) accumulation at early stage of the seedling development by the petri dish germination method indoors. The results showed that the inhibitory effect of PTIO or ABA on the embryo germination was relieved by the SNP pretreatment under light. However, the SNP pretreatment did not influence the inhibitory action of NBD to embryo germination. The SNP pretreatment increased the chlorophyll content in leaves of seedlings, which was not removed by PTIO treatment. The contents of hydrogen peroxide (H₂O₂) and superoxide anion in seedling leaves were increased by SNP pretreatment, which was cancelled by PTIO treatment. The PTIO treatment reduced the superoxide anion content in seedling roots, which was removed by SNP pretreatment. The results would lay the foundation for interpreting the relationships between reactive nitrogen species (RNS) mediated (NO-dependent) dormancy removal and ROS (H₂O₂ and superoxide anion) accumulation in the embryo germination.

Key words: Sorbus pohuashanensis, embryo germination, nitric oxide, abscisic acid, ethylene

中图分类号:

S718.43

杨玲, 王佳男, 边磊, 沈海龙. 外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响[J]. 林业科学, 2013, 49(6): 60-67.

Yang Ling, Wang Jianan, Bian Lei, Shen Hailong. Effects of Exogenous Nitric Oxide on Embryo Germination and ROS Accumulation in Seedling Growth Initial Stage of Sorbus pohuashanensis[J]. Scientia Silvae Sinicae, 2013, 49(6): 60-67.

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外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响

Effects of Exogenous Nitric Oxide on Embryo Germination and ROS Accumulation in Seedling Growth Initial Stage of Sorbus pohuashanensis

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