关键词: 杜仲, 转录组, SSR, 引物

Abstract: To study the genetic diversity of Eucommia ulmoides without information of the whole genome, the SSR primers were designed based on the transcriptome sequencing data (unpublished) from leaves and fruits of E. ulmoides. The SSR loci were analyzed using microsatellite locus scan tool SSRIT to analyze 49 610 sequences, and we screened out 1 442 SSR loci which distributed in 1 334 sequences, accounting for 2.9% of the transcriptome sequences. The dinucleotide repeat is the most abundant repeat type, accounting for 69.90% of the total number of SSRs. We observed 150 kinds of repeating units and found that the highest frequency is AG/TC, accounting for 32.73% of the total number of SSRs. Additionally, we found a small amount of CG repeats. A total 85 pairs of primers were designed and synthesized, and the primers were verified by using 8 different excellent clones and among them 50 pairs of primers were able to amplify products, of which 20 pairs of primers were polymorphic. This study had an important application value to analyze genetic diversity of E. ulmoides by using SSR molecular markers.

Key words: Eucommia ulmoides, transcriptome, SSR, primer