3种生物农药对悬铃木方翅网蝽的室内毒力及解毒酶活性的影响

doi:10.11707/j.1001-7488.20120714

林业科学 ›› 2012, Vol. 48 ›› Issue (7): 86-91.doi: 10.11707/j.1001-7488.20120714

3种生物农药对悬铃木方翅网蝽的室内毒力及解毒酶活性的影响

郝德君, 陆佳伟, 殷丽

南京林业大学森林资源与环境学院南京 210037

收稿日期:2011-01-16 修回日期:2011-04-22 出版日期:2012-07-25 发布日期:2012-07-25

Toxicity of Three Biopesticides and Their Effect on Detoxification Enzyme Activity of Corythucha ciliata

Hao Dejun, Lu Jiawei, Yin Li

College of Forest Resources and Environment, Nanjing Forestry University Nanjing 210037

Received:2011-01-16 Revised:2011-04-22 Online:2012-07-25 Published:2012-07-25

摘要/Abstract

摘要：

室内条件下采用浸叶法分别测定苦参碱、阿维菌素、苏云金芽孢杆菌(Bt)3种药剂对悬铃木方翅网蝽1,5龄若虫和成虫的毒力; 采用生化方法测定3种药剂对不同虫态悬铃木方翅网蝽的羧酸酯酶(CarE)、乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GST)活性影响。结果表明:3种药剂对方翅网蝽具有较好的毒杀效果,校正死亡率均随着浓度的增加而升高。1龄若虫对药剂的敏感性最强,5龄若虫和成虫的敏感性相近。3种药剂中苦参碱的毒力最高,对1,5龄若虫和成虫的LC₅₀分别为0.195 7,194.9和197.3 mg ·L^-1; 其次为阿维菌素,对不同虫态的LC₅₀分别为10.66,420.5和472.0 mg ·L^-1; 苏云金杆菌对不同虫态的LC₅₀分别为3.23, 1 414.2和6 967.2 mg ·L^-1。3种药剂处理后,悬铃木方翅网蝽不同虫态的羧酸酯酶、乙酰胆碱酯酶和谷胱甘肽S-转移酶活性均产生不同程度的诱导效应。以苏云金杆菌处理的解毒酶活性增强最明显, 1,5龄若虫和成虫的3种解毒酶活性均明显高于对照; 其次为阿维菌素处理,3种虫态的谷胱甘肽S-转移酶活性, 1龄若虫的乙酰胆碱酯酶活性和成虫的羧酸酯酶活性明显高于对照; 苦参碱处理的解毒酶活性增强最不明显,仅引起3种虫态的谷胱甘肽S-转移酶活性、1龄若虫的乙酰胆碱酯酶活性较对照显著增强。1,5龄若虫和成虫不同虫态相比,药剂处理后的5龄若虫体内解毒酶活性高于成虫和1龄若虫。

关键词: 悬铃木方翅网蝽, 生物农药, 毒力测定, 羧酸酯酶, 乙酰胆碱酯酶, 谷胱甘肽S-转移酶

Abstract:

Toxicities of matrine, avermectin and Bacillus thuringiensis (Bt) against Corythucha ciliata were evaluated in laboratory by leaf-dip method. The results showed that matrine had the highest toxicity among the three reagents, and the LC₅₀to lst nymph, 5th nymph and adults of C. ciliata were 0.195 7, 194.9 and 197.3 mg·L^–1, respectively. The LC₅₀ of avermectin for three ages’ insects were 10.66, 420.5 and 472.0 mg·L^–1, and that of BT were 3.23,1 414.2 and 6 967.2 mg·L^–1. The influence of three pesticides on detoxification enzymes was also detected and the results indicated the activities of carboxylesterase (CarE), acetylcholinesterase (AChE) and glutathione-s-transferase (GST) in the BT-treated insects were significantly higher than those in the control. The GST activity of three development stages, the CarE activity of adult and the AchE activity of lst nymph with avermectin treatment were significantly higher than the control. The GST activity of three development stages and the AchE activity of lst nymph treated with matrine were significantly higher than the control. The activities of CarE, AChE and GST were different among development stages of C. ciliata.

Key words: Corythucha ciliata, biological pesticide, toxicity determination, carboxylesterase, acetylcholinesterase, glutathione-s-transferase

中图分类号:

S767.3

郝德君;陆佳伟;殷丽. 3种生物农药对悬铃木方翅网蝽的室内毒力及解毒酶活性的影响[J]. 林业科学, 2012, 48(7): 86-91.

Hao Dejun;Lu Jiawei;Yin Li. Toxicity of Three Biopesticides and Their Effect on Detoxification Enzyme Activity of Corythucha ciliata[J]. Scientia Silvae Sinicae, 2012, 48(7): 86-91.

[1]	张慧春, 朱正阳, 郑加强, 周宏平, 唐进根. 面向林业病虫害防治的生物农药喷施系统[J]. 林业科学, 2018, 54(10): 116-124.
[2]	付宁宁, 刘佳, 渠成, 王然, 许奕华, 罗晨, 李峰奇. 悬铃木方翅网蝽化学感受蛋白CcilCSP1的结构及其结合寄主挥发物的预测分析[J]. 林业科学, 2017, 53(10): 109-117.
[3]	刘立宏, 王峰, 马玲, 王步勇, 陈俏丽, 刘晓晗, 董婉莹, 薛羿. 松材线虫乙酰胆碱酯酶1基因沉默及蛋白抑制剂[J]. 林业科学, 2015, 51(1): 66-73.
[4]	邹传山;曹传旺;张国财;王志英. 落叶松毛虫2种解毒酶的体躯分布特征及对3种杀虫剂敏感性比较[J]. 林业科学, 2013, 49(3): 165-169.
[5]	徐明, 刘冬梅, 徐福元, 施建华. Bt与灭幼脲混剂对美国白蛾第2,3代幼虫的联合毒力及防治效果[J]. 林业科学, 2013, 49(12): 171-174.
[6]	张雁;郭同斌;诸葛强. 转Bt基因南林895杨对杨扇舟蛾体内酶的影响[J]. 林业科学, 2012, 48(6): 95-99.
[7]	段丹丹;王有年;成军;招嘉宁;师光禄. 欧亚旋覆花提取物对朱砂叶螨生物活性及其体内几种酶活性的影响[J]. 林业科学, 2012, 48(4): 156-161.
[8]	杜娟;赵磊;师光禄;王有年;仝宝生. 牵牛子杀螨活性物质鉴定及其作用机制初探[J]. 林业科学, 2011, 47(11): 199-203.
[9]	王有年;李青;李照会;师光禄. 萹蓄提取物对朱砂叶螨的触杀活性及相关酶活性的影响[J]. 林业科学, 2010, 46(10): 103-107.
[10]	杨建霞　郝德君　周曙东　戴华国. 寄主植物对松墨天牛的营养效应及对体内酯酶与羧酸酯酶活性的影响[J]. 林业科学, 2009, 12(1): 97-101.
[11]	王福莲李传仁刘万学万方浩. 新入侵物种悬铃木方翅网蝽的生物学特性与防治技术研究进展*[J]. 林业科学, 2008, 44(6): 143-147.
[12]	王海香申照静杜娟王有年师光禄. 核桃青皮提取物对朱砂叶螨的毒力及相关酶活性的测定[J]. 林业科学, 2008, 44(5): 70-74.
[13]	郭同斌嵇保中诸葛强黄敏仁. 转基因杨树对杨小舟蛾幼虫解毒酶活性的影响[J]. 林业科学, 2007, 43(05): 59-63.
[14]	李会平黄大庄纪慧芳闫海霞张宏玉. 取食不同杨树对光肩星天牛幼虫体内羧酸酯酶活性的影响[J]. 林业科学, 2007, 43(04): 50-53.
[15]	操海群岳永德彭镇华花日茂汤锋. 竹提取物对棉铃虫幼虫体内几种酶系活性的影响[J]. 林业科学, 2006, 42(07): 145-148.

3种生物农药对悬铃木方翅网蝽的室内毒力及解毒酶活性的影响

Toxicity of Three Biopesticides and Their Effect on Detoxification Enzyme Activity of Corythucha ciliata

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式