关键词: 铁炮百合, MDHAR, 克隆, 表达

Abstract:

A all-length cDNA of LlMDHAR gene was cloned from Lilium longiflorum cv. ‘White Heaven’by RACE. The gene consisted of 1 502 bp encoding a protein of 434 amino acids. The sequencing indicated that the LlMDHAR gene shared highly homology with other plants. RT-PCR analysis showed that expression of LlMDHAR gene could be detected in the root, bulb and leaf, and the relative high expression occurred in the root and leaf. Under H₂O₂ stress, the expression of LlMDHAR gene was increased with the raising of H₂O₂ concentration.

Key words: Lilium longiflorum, monodehydroascorbate reductase gene (MDHAR), cloning, expression