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林业科学 ›› 2010, Vol. 46 ›› Issue (12): 176-181.doi: 10.11707/j.1001-7488.20101229

• 研究简报 • 上一篇    下一篇

甲基磺酸甲酯处理的豫杂一号泡桐丛枝病幼苗的生长及SSR分析

翟晓巧, 曹喜兵, 范国强   

  1. 河南农业大学泡桐研究所 郑州 450002
  • 收稿日期:2009-08-02 修回日期:2009-11-09 出版日期:2010-12-25 发布日期:2010-12-25
  • 通讯作者: 范国强

Growth of Paulownia Witches’Broom Seedlings Treated with Methylmethane Sulphonate and SSR Analysis

Zhai Xiaoqiao, Cao Xibing, Fan Guoqiang   

  1. Institute of Paulownia, Henan Agricultural University Zhengzhou 450002
  • Received:2009-08-02 Revised:2009-11-09 Online:2010-12-25 Published:2010-12-25

关键词: 豫杂一号泡桐, 丛枝病, 甲基磺酸甲酯, 形态变化, SSR

Abstract:

Effects of methyl methane sulphonate (MMS) on the morphological changes of the seedlings of Paulownia tomentosa × P.fortunei with witches’ broom and the phytoplasma existence were investigated. The treated seedlings were analyzed with SSR method. The results indicated that MMS treatment was able to morphologically eliminate the diseased symptom of the diseased seedlings, but growth of the seedlings decreased if MMS concentration was over 60 mg ·L-1. Moreover, there still existed the phytoplasma in the infected seedlings after being treated with 20 mg ·L-1 MMS, and the digestion sites of those DNA from the healthy, diseased and the diseased seedlings treated with 20 and 100 mg ·L-1 MMS with SSR analysis were all the same.

Key words: Paulownia tomentosa×P. fortunei;witches&rsquo, broom;methyl methane sulphonate(MMS);morphological change;SSR

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