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林业科学 ›› 2004, Vol. 40 ›› Issue (1): 52-57.doi: 10.11707/j.1001-7488.20040109

• 论文及研究报告 • 上一篇    下一篇

麦芽糖、NAA及ABA对华北落叶松体细胞胚成熟及生根的影响

齐力旺 韩一凡 韩素英 王建华 DietrichEwald   

  1. 中国林业科学研究院林业研究所,北京100091;德国联邦林业研究中心,林木育种与生物技术研究所EberswalderChaussee6,15377
  • 收稿日期:2003-06-30 修回日期:1900-01-01 出版日期:2004-01-25 发布日期:2004-01-25

Effects of Maltose,NAA and ABA on Somatic Maturation and Radicle Rooting of Larix principis-rupprechtii

Qi Liwang,Han Yifan,Han Suying,Wang Jianhua,Dietrich Ewald   

  1. Research Institute of Forestry, Chinese Academy of Forestry Beijing100091;Federal Research Centre for Forestry, Institute for Forestry Tree Breeding and Biotechnology Eberswalder Chaussee 6,15377 Waldsieversdorf,Germany
  • Received:2003-06-30 Revised:1900-01-01 Online:2004-01-25 Published:2004-01-25

摘要:

研究了麦芽糖、NAA、ABA对华北落叶松体细胞胚发生及体细胞胚根发生和再生完整植株的影响。结果表明:在胚性愈伤组织继代、增殖过程中,以0 5mg·L-1 的NAA代替2 ,4 -D后,有利于体细胞胚的成熟和根发生。在ABA 16mg·L-1 时,产生的华北落叶松体细胞胚,能够再生良好植株,ABA大于2 0mg·L-1 时对体细胞胚根的发育不利。在含3%麦芽糖、4 %PEG4 000活性炭1g·L--1的成熟培养基上,华北落叶松每克愈伤组织子叶胚达106.7个,生根率达57.89% ;显著地提高了成熟体细胞胚及其根的质量。

关键词: 华北落叶松, 体细胞胚, 麦芽糖, 下胚轴

Abstract:

In order to improve quality of somatic embryos and rooting frequency in Larix principis-rupprechtii,2,4-dichlorophenoxy acetic acid(2,4-D) was replaced by naphthalene acetic acid(NAA),which improved the quality of early stage embryos, and thus improved the formation of high quality somatic embryos. Subcultures on this maturation medium, containing 3% maltose to replace sucrose, together with 4% PEG4000 and 0.1% activated charcoal (AC), the maturation frequency of somatic embryos was not lower (106.7 embryos per gram embryogenic calli, EC), but the quality of somatic embryos was improved distinctly, such as the hypocotyl was longer and the rooting frequency was up to 57.89%. The root system formed grew normal. Woody plant medium (WPM) as germination medium for somatic embryos, containing phloroglucinol (PG) 6 mg·L-1 was the best concerning embryo germination and rooting.

Key words: Larix principis-rupprechtii, Somatic embryos, Maltose, Hypocotyl