固氮豆科树种和豆科树种根瘤菌资源的研究

林业科学 ›› 1996, Vol. 32 ›› Issue (5): 434-440.

固氮豆科树种和豆科树种根瘤菌资源的研究

韩素芬

南京林业大学南京 210037

收稿日期:1995-05-26 出版日期:1996-09-25 发布日期:1996-09-25
基金资助:
国家自然科学基金资助项目(39170642)

A STUDY ON NATURAL RESOURCES OF NODULATING TREE LEGUMES AND STRAINS OF RHIZOBIA

Sufen Han

Nanjing Forestry University Nanjing 210037

Received:1995-05-26 Online:1996-09-25 Published:1996-09-25

摘要/Abstract

摘要：

调查观察了48属122种豆科树种的结瘤情况,其中82种结瘤。采用Vincent JM分离方法^[1],从根瘤中分离获得了123个根瘤菌株的纯培养,各菌株的菌落特性和生长速度差异明显.经回接和交叉接种试验进一步确定它们为豆科树种根瘤菌。

关键词: 豆科树种, 根瘤菌, 结瘤, 共生固氮

Abstract:

48 genera 122 species of tree legumes were examined for nodulation in field and seedling nursery. Among them, 82 species were found to be nodulated. 123 strains of root nodule bacteria from nodules of the trees were isolated following the method suggested by Vincent (1970). Isolates of rhizobia were purified and maintained on YEMA slants. Strains of rhizobia obviously differ from one another in characteristics of colonies and growth rate.

Key words: Tree legumes, Root nodule bacteria, Nodulation, Symbiotic nitrogen fixation

韩素芬. 固氮豆科树种和豆科树种根瘤菌资源的研究[J]. 林业科学, 1996, 32(5): 434-440.

Sufen Han. A STUDY ON NATURAL RESOURCES OF NODULATING TREE LEGUMES AND STRAINS OF RHIZOBIA[J]. Scientia Silvae Sinicae, 1996, 32(5): 434-440.

图/表 1

参考文献 9

1	Vincent J M, "A Manual for The Practical Study of The Root Nodule Bacteria" IBP Handbook NO.15, Ox-ford: Blackwell Scientific Publications, 1970
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3	周湘泉豆类植物共生固氮研究近况和展望南京林产工业学院学报, 1983, 7 (4): 88- 99 doi: 10.3969/j.jssn.1000-2006.1983.04.008
4	Allen O N, and Allen E K. The Leguminose, A Source Book of Characteristics, Uses, and Nodulation. Madison The University of Wisconsin, 1981
5	Basak M K. and Goyel S K. Studies on Tree Legumes Ⅱ. Further Additions to The List of Nodulating Tree Legumes. Plant and soil, 1980, 56(1): 33-37
6	周湘泉 , 韩素芬豆科树种根瘤菌共生体系的研究I.结瘤观察、分离、回接和交叉接种南京林学院学报, 1984, (2): 32- 42 doi: 10.3969/j.jssn.1000-2006.1984.02.005
7	韩素芬 , 周湘泉我国豆科树种结瘤情况南京林业大学学报, 1990, 14 (3): 84- 90 doi: 10.3969/j.jssn.1000-2006.1990.03.016
8	Cleyet-marel J C. Procedure for sampling and Preserving nodules harvested on Legume roots. Technical handbook on symbiotic nitrogen fixation。Food and Agriculture Organization of The United Nations, Rome, 1983
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