Abstract: This paper is a study on chromosome banding technique of G-band. The somatic cells (root tip) of Cunninghamia lanceolata are used. After treated by the colchicine and fixed, the roots are zymolysed, dissociated and made into cell suspend material. They are, then, titrated and dried, throwgh the procedure 0.2 N HCL→0.00175M NaOH→(NH₄)Fe(SO₄)₂·12 H₂O→Giemsa, the G-band are shown.Afterwards, Pinus massoniana and Aleurites fordii are used respectively in the experiment, the same result are gotten as well.