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林业科学 ›› 2013, Vol. 49 ›› Issue (6): 90-96.doi: 10.11707/j.1001-7488.20130613

• 论文与研究报告 • 上一篇    下一篇

2个欧美杨品种树皮内生真菌多样性及优势种群动态变化

李永1,2, 朴春根2, 贺伟1, 常聚普3, 王海明4, 郭立民3, 谢守江3, 郭民伟2   

  1. 1. 北京林业大学林学院 北京 100083;
    2. 中国林业科学研究院森林生态环境与保护研究所 北京 100091;
    3. 濮阳市林业科学院 濮阳 457000;
    4. 菏泽市林木保护站 菏泽 274002
  • 收稿日期:2012-12-19 修回日期:2013-01-30 出版日期:2013-06-25 发布日期:2013-07-16
  • 通讯作者: 贺伟
  • 基金资助:

    林业公益性行业专项(201104054);中国林业科学研究院森林生态环境与保护研究所院所基金(CAFRIFEEP201102-4); 林业微生物资源服务与共享(NIMR-2011-7)。

Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Populus×euramericana

Li Yong1,2, Piao Chungen2, He Wei1, Chang Jupu3, Wang Haiming4, Guo Limin3, Xie Shoujiang3, Guo Minwei2   

  1. 1. College of forest, Beijing Forestry University Beijing 100083;
    2. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry Beijing 100091;
    3. Puyang Academy of Forestry Puyang 457000;
    4. Heze Forest Protection Station Heze 274002
  • Received:2012-12-19 Revised:2013-01-30 Online:2013-06-25 Published:2013-07-16

摘要:

利用组织分离法从欧美杨2品种欧美杨107杨、中林46杨中(996块组织)分离内生真菌1 252株, 107杨和中林46杨分别分离出645株和607株。利用形态特征和分子生物学方法鉴定为17个属、32个分类单元, 包括担子菌2个分类单元,子囊菌30个分类单元均为子囊菌。在基于ITS构建的系统进代树上、2杨树品种内生菌均形成5个稳定的分支,包括在囊菌纲2个分支,包括散囊菌纲、类壳菌纲等。2个杨树品种内生真菌优势种群包括链格孢、葡萄座腔菌、镰孢菌、间座壳菌等,其中链格孢、葡萄座腔菌是最为常见的优势种群,2品种内生菌的优势种群会随季节变化而发生变化。

关键词: 内生真菌, 欧美杨, 树皮, 多样性

Abstract:

In order to understand predominant species, seasonal dynamic and diversity of fungal endophytes in barks of Populus×euramericana cv.‘74/76’ and P.×euramericana cv. ‘Zhonglin46’,fungal endophytes were isolated from the barks by tissue isolation method, and identified by sequence analysis of the internal transcribed spacer and by morphological characteristics of microscopic observation. A total of 1 252 fungal endophytes were isolated from 996 bark tissues. The fungal endophytes were classified into 32 different fungal taxa belonging to 17 genera of Ascomycota. The predominant species included Alternaria alternata, Botryosphaeria dothidea, Fusarium spp., Diaporthe spp., among which A. alternata and B. dothidea were the most common predominant species in the barks. The predominant fungal endophytes in the bark tissue varied in different seasons.

Key words: fungal endophytes, Populus×euramericana, bark, diversity

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