关键词: 拟南芥, 尾翼茎突变体, 木材形成, 分子标记, 基因定位

Abstract:

Arabidopsis thaliana undergoes to a certain degree secondary growth under short day conditions, thus it can be used to study wood formation. In our previous work, we screened a mutant (tail-fins-on-stem, tfos) that displayed unique characteristics under short day conditions, such as shorter stem with a few ridge-like structures on the middle and the basal stem,slower growth and twisted rosette leaves with serrated margin, in comparison with the wild type. Microscopic observation showed that there was vascular structure in the middle of the ridge, likely due to abnormal differentiation of the cells in the stem. Genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene. Further analysis, with molecular markers, showed that the mutant gene was mapped on the Chromosome I and displayed co-separation with the SSLP marker F11A17- 48074. This study paved a way for cloning the gene controlling the phenotypes.

Key words: Arabidopsis thaliana, tfos, wood formation, molecular marker, gene location