欢迎访问林业科学,今天是

林业科学 ›› 2012, Vol. 48 ›› Issue (3): 136-142.doi: 10.11707/j.1001-7488.20120323

• 研究简报 • 上一篇    下一篇

脉冲磁场对松材线虫生理生化及超微结构的影响

张明1,2, 宋晓斌2, 张星耀1, 王振朝3, 梁军1   

  1. 1. 中国林业科学研究院森林生态环境与保护研究所 国家林业局森林保护学重点实验室 北京 100091;2. 西北农林科技大学林学院 杨凌 712100;3. 河北大学电信学院 保定 071002
  • 收稿日期:2011-05-24 修回日期:2011-09-12 出版日期:2012-03-25 发布日期:2012-03-25
  • 通讯作者: 梁军

Effects of Pulsed Magnetic Field on the Biochemical and Physiological Characters and Ultrastructure of Pine Wood Nematode

Zhang Ming1,2, Song Xiaobin2, Zhang Xingyao1, Wang Zhenchao3, Liang Jun1   

  1. 1. Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091;2. College of Forestry, Northwest A&F University Yangling 712100;3. College of Electronic and Information Engineering, Hebei University Baoding 071002
  • Received:2011-05-24 Revised:2011-09-12 Online:2012-03-25 Published:2012-03-25

关键词: 脉冲磁场, 脉冲前沿宽度, 纤维素酶活性, 死亡率, 松材线虫

Abstract:

In this paper, we employed the pulsed magnetic field (PMF) processing to investigate the effect of different pulse rise time and treatment time on the survival, reproduction, cellulase activity and surface characteristics of the pine wood nematode (PWN). The results showed that the wider pulse and the longer treatment time were able to reduce the survival, and inhibit the cellulase activity and reproduction of PWN. The 4th instar and females had a strong resistance to the pulsed magnetic field. With pulse rise time of 10 ms and the treatment of 60 min, the adjusted mortality of PWN was 100%. After the PMF treatment, the cellulase activity of PWN increased within a short time, and then declined with the treatment time. The observation with a scanning electron micrograph (SEM) revealed that there was no significant change in morphology of PWN, but the quantity of rod-shaped bacteria carried by PWN was reduced.

Key words: pulsed magnetic field, pulse rise time, cellulase activity, mortality rate, pine wood nematode

中图分类号: