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林业科学 ›› 2010, Vol. 46 ›› Issue (4): 64-69.doi: 10.11707/j.1001-7488.20100410

• 论文 • 上一篇    下一篇

猕猴桃冷藏期不同组织区光合色素、叶绿体细胞学及其光合放氧的变化

郭学民1,2,3 王贵禧1 高荣孚3   

  1. 1.中国林业科学研究院林业研究所 国家林业局林木培育重点实验室 北京100091; 2.河北科技师范学院生命科学系 秦皇岛066600; 3.北京林业大学生物科学与技术学院 北京100083
  • 收稿日期:2009-03-02 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25
  • 通讯作者: 王贵禧

Changes of Photosynthetic Pigments, Chloroplast Cytology and Its Photosynthetic Oxygen Evolution in Different Tissue Zone of Kiwifruit During Cold Storage Period

Guo Xuemin1,2,3,Wang Guixi1,Gao Rongfu3   

  1. 1.Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry, CAF Beijing 100091;2.Department of Life Science, Hebei Normal University of Science and Technology Qinhuangdao 066600;3.College of Life Science and Technology, Beijing Forestry University Beijing 100083
  • Received:2009-03-02 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25

摘要:

研究‘秦美’猕猴桃冷藏期外果肉、内果肉和中轴胎座3个组织区的光合色素、叶绿体细胞学及其光合放氧的变化特征。结果表明: ‘秦美’猕猴桃果实各组织区光合色素的组分随着果实的冷藏处于动态变化之中; 在冷藏各时期叶绿素(Chl)和(或)脱植基叶绿素的存在使果实始终呈现绿色; 同一组织区Chl a,Chl b和Chl(a+b)含量随着冷藏时间延长而逐渐减少,Chl a/b则上升; 在同一冷藏时期,各组织区Chl a,Chl b和Chl(a+b)含量高低为外果肉>内果肉>中轴胎座。冷藏开始,在显微镜明场下各组织区叶绿体均呈不规则球形,在荧光显微镜下则呈蜂窝状,一些淀粉粒从叶绿体中散落出来; 随着冷藏时间延长,各组织区叶绿体数量减少,体积变小,淀粉粒水解,片层结构崩解; 冷藏30天时,叶绿体淀粉粒数量明显变少,常见片层结构残迹。随着冷藏时间延长,各组织区光合放氧速率均下降,同一时期各冷藏时间光合放氧速率为外果肉>内果肉>中轴胎座。表明在冷藏过程中,猕猴桃各组织区的光合色素、叶绿体形态结构和光系统Ⅱ光合放氧是有差异的。

关键词: 猕猴桃, 果实组织区, 光合色素, 叶绿体, 光合放氧

Abstract:

The variation characteristics of photosynthetic pigments, chloroplast cytology and its photosynthetic oxygen evolution rate were investigated in outer pericarp (OP), inner pericarp (IP) and axile placenta (AP) of Actinidia deliciosa ‘Qinmei’ fruit during cold storage,obtain basic knowledge of quality keeping in process of cold storage. The results showed that the composition of photosynthetic pigments in each tissue zone changed with the time of refriferation. The presence of Chl and (or) chlide make the pulp assume green color; however with the fruit cold-stored time, Chl a, Chl b and Chl(a+b) contents decreased gradually and Chl a/b increased. At a given cold-stored stage, the orders of content of Chl a, Chl b and Chl (a+b) of each tissue zone of the fruit were OP>IP>AP. The chloroplasts were solid irregular sphericity under lightfield and honeycomb like under fluorescence microscope before cold storage. Some starch grains were spilled into the cytoplasm from the chloroplasts. The number and volume of the chloroplast of every tissue zone became less and smaller, starch grains hydrolyzed and chloroplasts disintegrated with cold storage. The starch grain became less significantly and remnant lamella structure was frequently found after 30 d cold storage. The photosynthetic oxygen evolution rates of each tissue zone decreased with cold storage and the orders of the rates were: OP>IP>AP at the same period of cold storage. It was concluded that there were differences among photosynthetic pigments, morphology structure of chloroplast and photosynthetic oxygen evolution rate in each tissue zone during cold storage.

Key words: kiwifruit, fruit tissue zone, photosynthetic pigment, chloroplast, photosynthetic oxygen evolution